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Relation between DNA damage measured by comet assay and OGG1 Ser326Cys polymorphism in antineoplastic drugs biomonitoring

2015-09Journal article Open access
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dc.contributor.authorLadeira, Carina
dc.contributor.authorViegas, Susana
dc.contributor.authorPádua, Mário
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorGomes, Manuel C.
dc.contributor.authorBrito, Miguel
dc.date.accessioned2016-08-23T16:07:07Z
dc.date.available2016-08-23T16:07:07Z
dc.date.issued2015-09
dc.description.abstractAntineoplastic drugs are hazardous chemical agents used mostly in the treatment of patients with cancer, however health professionals that handle and administer these drugs can become exposed and develop DNA damage. Comet assay is a standard method for assessing DNA damage in human biomonitoring and, combined with formamidopyrimidine DNA glycosylase (FPG) enzyme, it specifically detects DNA oxidative damage. The aim of this study was to investigate genotoxic effects in workers occupationally exposed to cytostatics (n = 46), as compared to a control group with no exposure (n = 46) at two Portuguese hospitals, by means of the alkaline comet assay. The potential of the OGG1 Ser326Cys polymorphism as a susceptibility biomarker was also investigated. Exposure was evaluated by investigating the contamination of surfaces and genotoxic assessment was done by alkaline comet assay in peripheral blood lymphocytes. OGG1 Ser326Cys (rs1052133) polymorphism was studied by Real Time PCR. As for exposure assessment, there were 121 (37%) positive samples out of a total of 327 samples analysed from both hospitals. No statistically significant differences (Mann-Whitney test, p > 0.05) were found between subjects with and without exposure, regarding DNA damage and oxidative DNA damage, nevertheless the exposed group exhibited higher values. Moreover, there was no consistent trend regarding the variation of both biomarkers as assessed by comet assay with OGG1 polymorphism. Our study was not statistically significant regarding occupational exposure to antineoplastic drugs and genetic damage assessed by comet assay. However, health professionals should be monitored for risk behaviour, in order to ensure that safety measures are applied and protection devices are used correctly.pt_PT
dc.identifier.citationLadeira C, Viegas S, Pádua M, Carolino E, Gomes MC, Brito M. Relation between DNA damage measured by comet assay and OGG1 Ser326Cys polymorphism in antineoplastic drugs biomonitoring. AIMS Genetics. 2015;2(3):204-18.pt_PT
dc.identifier.doi10.3934/genet.2015.3.204pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/6400
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAIMS Presspt_PT
dc.relation.publisherversionhttp://www.aimspress.com/article/10.3934/genet.2015.3.204pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectDNA damagept_PT
dc.subjectComet assaypt_PT
dc.subjectOGG1 polymorphismpt_PT
dc.subjectAntineoplastic drugspt_PT
dc.subjectOccupational exposurept_PT
dc.subjectBiomonitoringpt_PT
dc.titleRelation between DNA damage measured by comet assay and OGG1 Ser326Cys polymorphism in antineoplastic drugs biomonitoringpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage218pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage204pt_PT
oaire.citation.titleAIMS Geneticspt_PT
oaire.citation.volume2pt_PT
person.familyNameLadeira
person.familyNameViegas
person.familyNameCarolino
person.familyNameBrito
person.givenNameCarina
person.givenNameSusana
person.givenNameElisabete
person.givenNameMiguel
person.identifier144237
person.identifier248817
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.ciencia-idA919-7318-63DC
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0001-5588-0074
person.identifier.orcid0000-0003-1015-8760
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridJ-2572-2012
person.identifier.ridI-4053-2012
person.identifier.ridF-1012-2015
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id36463788000
person.identifier.scopus-author-id35270591500
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery77930d39-ed34-44dc-a4a6-9bf833e5e688

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