Repository logo
 
Publication

Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death

2005-07Journal article Open access
Simple item page
dc.contributor.authorNolasco, Sofia
dc.contributor.authorBellido, Javier
dc.contributor.authorGonçalves, João
dc.contributor.authorZabala, Juan Carlos
dc.contributor.authorSoares, Helena
dc.date.accessioned2014-01-01T19:01:02Z
dc.date.available2014-01-01T19:01:02Z
dc.date.issued2005-07
dc.description.abstractMicrotubules are polymers of alpha/beta-tubulin participating in essential cell functions. A multistep process involving distinct molecular chaperones and cofactors produces new tubulin heterodimers competent to polymerise. In vitro cofactor A (TBCA) interacts with beta-tubulin in a quasi-native state behaving as a molecular chaperone. We have used siRNA to silence TBCA expression in HeLa and MCF-7 mammalian cell lines. TBCA is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. In MCF-7 cells, cell death was preceded by a change in cell shape resembling differentiation.por
dc.identifier.citationNolasco S, Bellido J, Gonçalves J, Zabala JC, Soares H. Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death. FEBS Lett. 2005;579(17):3515-24.por
dc.identifier.issn1873-3468
dc.identifier.urihttp://hdl.handle.net/10400.21/3045
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0014579305006290por
dc.subjectActin cytoskeletonpor
dc.subjectApoptosispor
dc.subjectCaspase 7por
dc.subjectCell cyclepor
dc.subjectGene silencingpor
dc.subjectMicrotubule-associated proteinspor
dc.subjectMicrotubulespor
dc.subjectMolecular chaperonespor
dc.subjectPhenotypepor
dc.subjectRNA, Small interferingpor
dc.subjectTubulinpor
dc.subjectβ-Tubulin cofactor Apor
dc.subjectMolecular chaperonepor
dc.titleTubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell deathpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3524por
oaire.citation.startPage3515por
oaire.citation.titleFEBS Letterspor
oaire.citation.volume579por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Tubulin cofactor A gene silencing in mammalian cells induces.pdf
Size:
646.47 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESTeSL - Artigos