Publicação
Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
| dc.contributor.author | Nolasco, Sofia | |
| dc.contributor.author | Bellido, Javier | |
| dc.contributor.author | Gonçalves, João | |
| dc.contributor.author | Zabala, Juan Carlos | |
| dc.contributor.author | Soares, Helena | |
| dc.date.accessioned | 2014-01-01T19:01:02Z | |
| dc.date.available | 2014-01-01T19:01:02Z | |
| dc.date.issued | 2005-07 | |
| dc.description.abstract | Microtubules are polymers of alpha/beta-tubulin participating in essential cell functions. A multistep process involving distinct molecular chaperones and cofactors produces new tubulin heterodimers competent to polymerise. In vitro cofactor A (TBCA) interacts with beta-tubulin in a quasi-native state behaving as a molecular chaperone. We have used siRNA to silence TBCA expression in HeLa and MCF-7 mammalian cell lines. TBCA is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. In MCF-7 cells, cell death was preceded by a change in cell shape resembling differentiation. | por |
| dc.identifier.citation | Nolasco S, Bellido J, Gonçalves J, Zabala JC, Soares H. Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death. FEBS Lett. 2005;579(17):3515-24. | por |
| dc.identifier.issn | 1873-3468 | |
| dc.identifier.uri | http://hdl.handle.net/10400.21/3045 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0014579305006290 | por |
| dc.subject | Actin cytoskeleton | por |
| dc.subject | Apoptosis | por |
| dc.subject | Caspase 7 | por |
| dc.subject | Cell cycle | por |
| dc.subject | Gene silencing | por |
| dc.subject | Microtubule-associated proteins | por |
| dc.subject | Microtubules | por |
| dc.subject | Molecular chaperones | por |
| dc.subject | Phenotype | por |
| dc.subject | RNA, Small interfering | por |
| dc.subject | Tubulin | por |
| dc.subject | β-Tubulin cofactor A | por |
| dc.subject | Molecular chaperone | por |
| dc.title | Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 3524 | por |
| oaire.citation.startPage | 3515 | por |
| oaire.citation.title | FEBS Letters | por |
| oaire.citation.volume | 579 | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
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