Repository logo
 
Publication

Expression of YAP4 in Saccharomyces cerevisiae under osmotic stress

dc.contributor.authorNevitt, Tracy
dc.contributor.authorPereira, Jorge
dc.contributor.authorAzevedo, Dulce
dc.contributor.authorGuerreiro, Paulo
dc.contributor.authorRodrigues-Pousada, Claudina
dc.date.accessioned2014-01-02T14:51:41Z
dc.date.available2014-01-02T14:51:41Z
dc.date.issued2004-04
dc.description.abstractYAP4, a member of the yeast activator protein (YAP) gene family, is induced in response to osmotic shock in the yeast Saccharomyces cerevisiae. The null mutant displays mild and moderate growth sensitivity at 0.4 M and 0.8 M NaCl respectively, a fact that led us to analyse YAP4 mRNA levels in the hog1 (high osmolarity glycerol) mutant. The data obtained show a complete abolition of YAP4 gene expression in this mutant, placing YAP4 under the HOG response pathway. YAP4 overexpression not only suppresses the osmosensitivity phenotype of the yap4 mutant but also relieves that of the hog1 mutant. Induction, under the conditions tested so far, requires the presence of the transcription factor Msn2p, but not of Msn4p, as YAP4 mRNA levels are depleted by at least 75% in the msn2 mutant. This result was further substantiated by the fact that full YAP4 induction requires the two more proximal stress response elements. Furthermore we find that GCY1, encoding a putative glycerol dehydrogenase, GPP2, encoding a NAD-dependent glycerol-3-phosphate phosphatase, and DCS2, a homologue to a decapping enzyme, have decreased mRNA levels in the yap4 -deleted strain. Our data point to a possible, as yet not entirely understood, role of the YAP4 in osmotic stress response.por
dc.identifier.citationNevitt T, Pereira J, Azevedo D, Guerreiro P, Rodrigues-Pousada C. Expression of YAP4 in Saccharomyces cerevisiae under osmotic stress. Biochem J. 2004;379(Pt 2):367-74.por
dc.identifier.issn1470-8728
dc.identifier.urihttp://hdl.handle.net/10400.21/3048
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherBiochemical Societypor
dc.relation.publisherversionhttp://www.biochemj.org/bj/379/0367/bj3790367.htmpor
dc.subjectDNA-Binding proteinspor
dc.subjectGene expression regulationpor
dc.subjectMitogen-activated protein kinasespor
dc.subjectMutationpor
dc.subjectNuclear proteinspor
dc.subjectOsmotic pressurepor
dc.subjectPhenotypepor
dc.subjectResponse elementspor
dc.subjectSaccharomyces cerevisiaepor
dc.subjectTranscription factorspor
dc.titleExpression of YAP4 in Saccharomyces cerevisiae under osmotic stresspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage374por
oaire.citation.startPage367por
oaire.citation.titleBiochemical Journalpor
oaire.citation.volume379por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Expression of YAP4 in Saccharomyces cerevisiae under osmotic stress.pdf
Size:
261.01 KB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:

Collections