Logo do repositório
 
Publicação

Cytotoxic activity of settled dust in primary schools: a multi-matrix, multi-season screening approach for children’s environmental health

2026-10Artigo científico Acesso aberto
Ver registo simples
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorCervantes, Renata
dc.contributor.authorPena, Pedro
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorSoszczyńska, Ewelina
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorViegas, Susana
dc.contributor.authorViegas, Carla
dc.date.accessioned2026-10-08T15:34:08Z
dc.date.available2026-10-08T15:34:08Z
dc.date.issued2026-10
dc.descriptionThe author(s) declared that financial support was received for this work and/or its publication. The authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020. This work is also supported by national funds through FCT/MCTES/FSE/UE, 2023.01366.BD (doi: 10.54499/2023.01366.BD) and 2025.02613.BD. This work was also supported by the Academy of Medical Sciences Springboard award Round 7 (SBF007\100130). Funded by the European Union (grant agreement: 101056883). InChildHealth is also receiving funding from the Swiss State Secretariat for Education, Research and Innovation (SERI grant agreement 22.00324), from the United Kingdom Research and Innovation (UKRI grant agreement 10040524), and from the Australian National Health & Medical Research Council (NHMRC grant agreements APP2017786 and APP2008813).
dc.description.abstractChildren spend up to nine hours daily in school environments, yet the potential health impact of accumulated indoor dust remains largely uncharacterised. This study provides the first multi-matrix, multi-season assessment of passive dust samples from Portuguese primary schools, using the MTT assay to evaluate the biological activity of complex indoor pollutant mixtures. 207 passive samples were collected across 11 primary schools in the Lisbon Metropolitan Area, using four sampling matrices, settled dust filters, electrostatic dust cloths (EDC), electrostatic dust cloths on T-shirts (EDCT), and floor mops, across six indoor locations, warm and cold seasons, and in urban and rural contexts. Cytotoxic activity was assessed in A549 (human alveolar epithelial) and SK (swine kidney) cells, potential azole resistance was detected by growth on azole-supplemented culture media. Cytotoxic activity was detected in 64.7% (A549) and 24.6% (SK) of samples. Sampling matrix was the strongest predictor of cytotoxic variability for both cell lines (A549: R2 = 0.199; SK: R2 = 0.081; p < 0.001), with floor mops exhibiting the highest cytotoxic rates (A549: 94.4%; SK: 83.3%). Season was a significant secondary modulator exclusively for A549 cells, with cold-season positivity of 82.8% versus 50.0% (p < 0.001). Geographic context, school identity, and indoor location showed no significant effects, suggesting cytotoxic variability reflects building-level operational factors, cleaning practices, and ventilation, rather than environmental setting. Spearman analysis revealed cell-line and compound-specific associations between fungal burden, potential azole resistance detected by growth on azole-supplemented culture media, and cytotoxic responses, with reduced susceptibility to itraconazole, voriconazole, and posaconazole detected in school dust isolates. These findings demonstrate that microbial contamination in school settled dust may result in potential health effects involving clinically relevant antifungal resistance species. Floor mop sampling is identified as a high-sensitivity sentinel approach for the combined biological and chemical toxic burden accumulated at floor level. Passive multi-matrix cytotoxicity assessment should be integrated into school indoor air quality surveillance to protect this vulnerable population.eng
dc.description.sponsorshipThe author(s) declared that financial support was received for this work and/or its publication. The authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020. This work is also supported by national funds through FCT/MCTES/FSE/UE, 2023.01366.BD (doi: 10.54499/2023.01366.BD) and 2025.02613.BD. This work was also supported by the Academy of Medical Sciences Springboard award Round 7 (SBF007\100130). Funded by the European Union (grant agreement: 101056883). InChildHealth is also receiving funding from the Swiss State Secretariat for Education, Research and Innovation (SERI grant agreement 22.00324), from the United Kingdom Research and Innovation (UKRI grant agreement 10040524), and from the Australian National Health & Medical Research Council (NHMRC grant agreements APP2017786 and APP2008813).
dc.identifier.citationCervantes R, Pena P, Carolino E, Soszczyńska E, Viegas S, Viegas C, et al. Cytotoxic activity of settled dust in primary schools: a multi-matrix, multi-season screening approach for children’s environmental health. Front Public Health. 2026;14:1946465.
dc.identifier.doi10.3389/fpubh.2026.1946465
dc.identifier.issn2296-2565
dc.identifier.urihttp://hdl.handle.net/10400.21/23213
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media SA
dc.relation.hasversionhttps://www.frontiersin.org/journals/public-health/articles/10.3389/fpubh.2026.1946465/full
dc.relation.ispartofFrontiers in Public Health
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEnvironmental health
dc.subjectChildren
dc.subjectCytotoxicity
dc.subjectFungal contamination
dc.subjectIndoor air
dc.subjectAir quality
dc.subjectPassive sampling
dc.subjectSchool environment
dc.subjectSettled dust
dc.subjectFCT_UIDB/05608/2020
dc.subjectFCT_UIDP/05608/2020
dc.titleCytotoxic activity of settled dust in primary schools: a multi-matrix, multi-season screening approach for children’s environmental healtheng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage1946465
oaire.citation.titleFrontiers in Public Health
oaire.citation.volume14
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameWellenkamp de Carvalho Freire de Cervantes
person.familyNamePena
person.familyNameCarolino
person.familyNameViegas
person.familyNameViegas
person.givenNameRenata
person.givenNamePedro
person.givenNameElisabete
person.givenNameSusana
person.givenNameCarla
person.identifier2041141
person.identifier248817
person.identifier.ciencia-id6018-3CC9-B640
person.identifier.ciencia-idD71B-656F-BB9D
person.identifier.ciencia-idA919-7318-63DC
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.orcid0000-0001-5990-4168
person.identifier.orcid0000-0002-2188-9318
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.orcid0000-0002-1545-6479
person.identifier.ridI-4053-2012
person.identifier.ridB-7217-2013
person.identifier.scopus-author-id57222077827
person.identifier.scopus-author-id35270591500
person.identifier.scopus-author-id55443609700
relation.isAuthorOfPublication28678633-1f6a-4b79-8150-12a857f4a531
relation.isAuthorOfPublicationf830ce64-7497-4ca0-bdc9-d1891a740a61
relation.isAuthorOfPublication31223136-1531-4060-aec7-4d3fc8529916
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication.latestForDiscovery28678633-1f6a-4b79-8150-12a857f4a531

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
Cytotoxic activity of settled dust in primary schools_a multi-matrix, multi-season screening approach for children’s environmental health.pdf
Tamanho:
1.2 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
4.03 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

ESSL - Artigos