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Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies

2018-10Journal article Open access
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dc.contributor.authorHenriques, Joana
dc.contributor.authorFale, Pedro
dc.contributor.authorPacheco, Rita
dc.contributor.authorFlorêncio, Maria Helena
dc.contributor.authorSerralheiro, Maria Luisa
dc.date.accessioned2018-11-27T14:41:14Z
dc.date.available2018-11-27T14:41:14Z
dc.date.issued2018-10
dc.description.abstractChemical compounds from leaves of fruit-producing trees, a waste from agricultural activity can be isolated and used as a source of natural bioactive chemicals. Boiling water was used as an extractant of bioactive compounds from Actinidia deliciosa leaves and co-extracted fibres were removed with ethanol precipitation. Rutin and quercitrin were the main flavonoids identified and quantified by RP-HPLC-DAD. No cytotoxicity was detected for any of the extracts towards Caco- 2 cell line. A permeation of approx. 14% of extract components through the cells monolayer was determined. The cell protein profile of Caco-2 cells was modified when in the presence of the fibre-free extract and transketolase was the protein over-expressed in the presence of polyphenols. Acetylcholinesterase inhibitory activity was also studied, IC50 of 0.56 mg/mL was obtained with the fibre-free extract. A. deliciosa leaves are a good source of phenolic compounds and, therefore, some advantage may be taken of this agricultural residue, due to their biological activity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHENRIQUES, Joana; [et al] – Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies. Journal of King Saud University Science. ISSN 1018-3647. Vol. 30, N.º 4 (2018), pp. 513-518pt_PT
dc.identifier.doihttps://doi.org/10.1016/j.jksus.2017.07.007pt_PT
dc.identifier.issn1018-3647
dc.identifier.issn2213-686X
dc.identifier.urihttp://hdl.handle.net/10400.21/9093
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://reader.elsevier.com/reader/sd/pii/S1018364717302057?token=E59FDCE4C7D8B274CBFC1905E979EA8FE121C6AFB9E0413BEA6092A0416FEE97A5089C0DAC3C27BA29BCE4B94182E26Ept_PT
dc.subjectActinidia deliciosa leavespt_PT
dc.subjectRutinpt_PT
dc.subjectQuercitrinpt_PT
dc.subjectAcetylcholinesterasept_PT
dc.subjectTransketolasept_PT
dc.subjectPolyphenols permeationpt_PT
dc.subjectCaco-2 cellspt_PT
dc.titlePhenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FQUI%2FUI0612%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00612%2F2013/PT
oaire.citation.endPage518pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage513pt_PT
oaire.citation.titleJournal of King Saud University Sciencept_PT
oaire.citation.volume30pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNameFale
person.familyNamePacheco
person.familyNameFlorêncio
person.familyNameSerralheiro
person.givenNamePedro
person.givenNameRita
person.givenNameMaria Helena
person.givenNameMaria Luisa
person.identifier1022927
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.ciencia-id2912-CC47-39F4
person.identifier.orcid0000-0002-4644-7655
person.identifier.orcid0000-0001-5192-3006
person.identifier.orcid0000-0001-5319-9058
person.identifier.orcid0000-0001-7541-9613
person.identifier.ridA-5570-2013
person.identifier.ridC-3062-2012
person.identifier.ridB-5404-2011
person.identifier.ridE-3407-2012
person.identifier.scopus-author-id25649775000
person.identifier.scopus-author-id8853708300
person.identifier.scopus-author-id6701856949
person.identifier.scopus-author-id6602688573
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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