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Orientador(es)
Resumo(s)
Chemical compounds from leaves of fruit-producing trees, a waste from agricultural activity can be isolated and used as a source of natural bioactive chemicals. Boiling water was used as an extractant of bioactive compounds from Actinidia deliciosa leaves and co-extracted fibres were removed with ethanol precipitation. Rutin and quercitrin were the main flavonoids identified and quantified by RP-HPLC-DAD. No cytotoxicity was detected for any of the extracts towards Caco- 2 cell line. A permeation of approx. 14% of extract components through the cells monolayer was determined. The cell protein profile of Caco-2 cells was modified when in the presence of the fibre-free extract and transketolase was the protein over-expressed in the presence of polyphenols. Acetylcholinesterase inhibitory activity was also studied, IC50 of 0.56 mg/mL was obtained with the fibre-free extract. A. deliciosa leaves are a good source of phenolic compounds and, therefore, some advantage may be taken of this agricultural residue, due to their biological activity.
Descrição
Palavras-chave
Actinidia deliciosa leaves Rutin Quercitrin Acetylcholinesterase Transketolase Polyphenols permeation Caco-2 cells
Contexto Educativo
Citação
HENRIQUES, Joana; [et al] – Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies. Journal of King Saud University Science. ISSN 1018-3647. Vol. 30, N.º 4 (2018), pp. 513-518
Editora
Elsevier