Repository logo
 
Publication

Complex Dynamics of defective interfering baculoviruses during serial passage in insect cells

2013Journal article Open access
Simple item page
dc.contributor.authorZwart, Mark P.
dc.contributor.authorPijlman, Gorben P.
dc.contributor.authorSardanyés, Josep
dc.contributor.authorDuarte, Jorge
dc.contributor.authorJanuário, Cristina
dc.contributor.authorElena, Santiago F.
dc.date.accessioned2015-10-09T17:18:37Z
dc.date.available2015-10-09T17:18:37Z
dc.date.issued2013
dc.description.abstractDefective interfering (DI) viruses are thought to cause oscillations in virus levels, known as the ‘Von Magnus effect’. Interference by DI viruses has been proposed to underlie these dynamics, although experimental tests of this idea have not been forthcoming. For the baculoviruses, insect viruses commonly used for the expression of heterologous proteins in insect cells, the molecular mechanisms underlying DI generation have been investigated. However, the dynamics of baculovirus populations harboring DIs have not been studied in detail. In order to address this issue, we used quantitative real-time PCR to determine the levels of helper and DI viruses during 50 serial passages of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) in Sf21 cells. Unexpectedly, the helper and DI viruses changed levels largely in phase, and oscillations were highly irregular, suggesting the presence of chaos. We therefore developed a simple mathematical model of baculovirus-DI dynamics. This theoretical model reproduced patterns qualitatively similar to the experimental data. Although we cannot exclude that experimental variation (noise) plays an important role in generating the observed patterns, the presence of chaos in the model dynamics was confirmed with the computation of the maximal Lyapunov exponent, and a Ruelle-Takens-Newhouse route to chaos was identified at decreasing production of DI viruses, using mutation as a control parameter. Our results contribute to a better understanding of the dynamics of DI baculoviruses, and suggest that changes in virus levels over passages may exhibit chaos.pt_PT
dc.identifier.citationZWART, Mark P.; [et al] – Complex Dynamics of defective interfering baculoviruses during serial passage in insect cells. Journal of Biological Physics. ISSN: 0092-0606. Vol. 39, nr. 2 (2013), pp. 327-342pt_PT
dc.identifier.doi10.1007/s10867-013-9317-9
dc.identifier.issn0092-0606
dc.identifier.issn1573-0689
dc.identifier.urihttp://hdl.handle.net/10400.21/5264
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationRubicon Grant from the Netherlands Organization for Scientific Researchpt_PT
dc.relationJCI-2011-10379pt_PT
dc.relationBotín Foundationpt_PT
dc.relationBFU2012-30805pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBaculoviruspt_PT
dc.subjectBifurcationspt_PT
dc.subjectChaospt_PT
dc.subjectDefective Interfering Viruspt_PT
dc.subjectExperimental Evolutionpt_PT
dc.titleComplex Dynamics of defective interfering baculoviruses during serial passage in insect cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage342pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage327pt_PT
oaire.citation.titleJournal of Biological Physicspt_PT
oaire.citation.volume39pt_PT
person.familyNameDuarte
person.familyNameJanuário
person.givenNameJorge
person.givenNameCristina
person.identifier.ciencia-idBC1D-1E83-E2B2
person.identifier.orcid0000-0003-2641-3199
person.identifier.orcid0000-0002-6978-876X
person.identifier.ridG-7261-2011
person.identifier.scopus-author-id35310049800
person.identifier.scopus-author-id56526791400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4dbec82a-caa4-4662-b156-af73687e867f
relation.isAuthorOfPublication42a4023b-b4c4-4456-aaba-d97bf8c14d6a
relation.isAuthorOfPublication.latestForDiscovery4dbec82a-caa4-4662-b156-af73687e867f

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Complex dynamics of defective interfering baculoviruses.pdf
Size:
1.05 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEL - Matemática - Artigos