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Microtubule cytoskeleton perturbation induced by taxol and colchicine affects chaperonin containing TCP-1 (CCT) subunit gene expression in Tetrahymena cells

2001-11Journal article Open access
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dc.contributor.authorCasalou, Cristina
dc.contributor.authorCyrne, Luísa
dc.contributor.authorRosa, Mónica Roxo
dc.contributor.authorSoares, Helena
dc.date.accessioned2018-09-21T12:58:54Z
dc.date.available2018-09-21T12:58:54Z
dc.date.issued2001-11
dc.description.abstractWe report the existence of a CCTϵ subunit gene that encodes subunit ϵ of the chaperonin CCT (chaperonin containing TCP-1) in Tetrahymena pyriformis. This work focuses on the study of the effects of the microtubule polymerizing agent taxol and the depolymerizing agent colchicine on microtubule dynamics and their role in the regulation of tubulin and CCT subunit genes. Under taxol treatment some TpCCT and tubulin genes are distinctly expressed until 30 min of treatment. Cytoplasmic TpCCT mRNA levels slightly decrease while tubulin transcripts are increasing. In colchicine treated cells TpCCT and tubulin transcripts decrease in the initial 30 min of treatment and then start to increase. However, both antimitotic agents induce TpCCT and tubulin gene transcription. This induction does not correlate with increased steady-state levels of TpCCT proteins and seems to be necessary to replete cytoplasmic TpCCT mRNAs. Moreover, we found that TpCCTϵ and TpCCTα but not TpCCTη are present in the insoluble fraction after a postmitochondrial fractionation that contains components of the ciliate cortex structure, basal bodies and cilia. This suggests that some TpCCT subunits may be associated with these structures. The association of TpCCTϵ subunit is stimulated either by taxol or colchicine treatment. These observations support the idea that CCT subunits could have additional roles in vivo.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCasalou C, Cyrne L, Rosa MR, Soares H. Microtubule cytoskeleton perturbation induced by taxol and colchicine affects chaperonin containing TCP-1 (CCT) subunit gene expression in Tetrahymena cells. Biochim Biophys Acta. 2001;1522(1):9-21.pt_PT
dc.identifier.doi10.1016/S0167-4781(01)00294-9pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/8878
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0167478101002949pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectChaperonin CCTpt_PT
dc.subjectMicrotubulept_PT
dc.subjectTaxolpt_PT
dc.subjectColchicinept_PT
dc.subjectTetrahymenapt_PT
dc.subjectGene expression regulationpt_PT
dc.subjectGenetic regulationpt_PT
dc.subjectSequence alignmentpt_PT
dc.subjectMolecular sequence datapt_PT
dc.titleMicrotubule cytoskeleton perturbation induced by taxol and colchicine affects chaperonin containing TCP-1 (CCT) subunit gene expression in Tetrahymena cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage21pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage9pt_PT
oaire.citation.titleBBA - Biochimica et Biophysica Actapt_PT
oaire.citation.volume1522pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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