Analyses approaches for bacteria

Ribeiro, Edna; Faria, Isabel

Publication

Analyses approaches for bacteria

2017-10Book part

dc.contributor.author	Ribeiro, Edna
dc.contributor.author	Faria, Isabel
dc.date.accessioned	2017-10-20T14:35:24Z
dc.date.available	2017-10-20T14:35:24Z
dc.date.issued	2017-10
dc.description.abstract	Bacterial bioburden assessments, particularly in the context of human environmental/occupational exposure, have major implications for public health risk assessment. Several sampling methods, which must be adapted to the studied environmental context, are currently available. Culture-dependent and culture-independent methodologies have been utilized for the analysis of bacterial communities in various environments. Culture-dependent techniques drove extraordinary advances in microbiology and allow the enhancement of bacterial material to be utilized in a supplementary analysis; however, these approaches may underestimate the bacterial bioburden of the studied samples. On the other hand, culture-independent approaches are considered more sensible and efficient with the capacity to provide valuable information regarding bacterial diversity and quantity; nevertheless, preferential amplification and poor primers specificity can account for major limitations. In order to perform valuable and efficient assessments of bacterial bioburden booth approaches should be utilized simultaneously.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Ribeiro E, Faria I. Analyses approaches for bactéria. In: Viegas C, Viegas S, Gomes AQ, Täubel M, Sabino R, editors. Exposure to microbiological agents in indoor and occupational environments. Springer; 2017. p. 97-108.	pt_PT
dc.identifier.doi	10.1007/978-3-319-61688-9_5	pt_PT
dc.identifier.isbn	978-3-319-61688-9
dc.identifier.uri	http://hdl.handle.net/10400.21/7433
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Springer Publishing Company	pt_PT
dc.relation.publisherversion	https://link.springer.com/chapter/10.1007/978-3-319-61688-9_5	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	pt_PT
dc.subject	Bacterial bioburden	pt_PT
dc.subject	Environmental sampling	pt_PT
dc.subject	Culture-dependent bacterial analysis	pt_PT
dc.subject	Culture-independent bacterial analysis	pt_PT
dc.title	Analyses approaches for bacteria	pt_PT
dc.type	book part
dspace.entity.type	Publication
oaire.citation.endPage	108	pt_PT
oaire.citation.startPage	97	pt_PT
person.familyName	Ribeiro
person.givenName	Edna
person.identifier.ciencia-id	C414-CDF2-D35A
person.identifier.orcid	0000-0003-1316-7750
rcaap.rights	restrictedAccess	pt_PT
rcaap.type	bookPart	pt_PT
relation.isAuthorOfPublication	a571bf34-bcda-49ca-b5cb-4cdecbb3d9c7
relation.isAuthorOfPublication.latestForDiscovery	a571bf34-bcda-49ca-b5cb-4cdecbb3d9c7