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Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilms

2023-10Journal article Open access
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dc.contributor.authorZegre, Miguel
dc.contributor.authorBarros, J.
dc.contributor.authorRibeiro, I. A.
dc.contributor.authorSantos, C.
dc.contributor.authorAranha Caetano, Liliana
dc.contributor.authorGonçalves, L.
dc.contributor.authorMonteiro, F.
dc.contributor.authorFerraz, M.
dc.contributor.authorBettencourt, A.
dc.date.accessioned2024-01-23T10:44:12Z
dc.date.available2024-01-23T10:44:12Z
dc.date.issued2023-10
dc.descriptionThe authors thank the Fundação para a Ciência e Tecnologia (FCT), Portugal for the financial support: projects UIDB/04138/2020 and UIDP/04138/2020 (iMed.Ulisboa), UIDB/00100/2020 (CQE), L. Gonçalves Principal Researcher grant (CEECIND/03143/2017), UIDB/ 05608/2020 and UIDP/05608/2020 (H&TRC).pt_PT
dc.description.abstractIntroduction: The treatment of bacterial joint and bone infections in patients after multiple revision arthroplasties is very challenging. An expanding number of studies report the co-isolation of fungal and bacterial species (such as Candida albicans and Staphylococcus aureus) from polymicrobial biofilm associated with infections related to bone infections. Current investigations establish that local-specific drug delivery scaffolds with low toxicity and increased efficiency to specific sites when compared to oral and systemic administration approaches, can considerably lower the number of viable microorganisms in polymicrobial biofilms, preventing simultaneously the progression of infection in bone disorder. Notably, the development of co-delivery systems of at least two antimicrobials is yet a neglected approach, while it may be a critical strategy for the treatment of infections associated with polymicrobial biofilms. Simultaneously, it is recommended to assess the contribution of each microbial population within the biofilm to select the best therapy to treat polymicrobial infections. Among different biomaterials used in scaffolds as drug-delivery carriers, poly(lactic acid) (PLA) based polymers are being widely studied due to their versatility, low toxicity, and tailored biodegradability having the US Food and Drug Administration approval for clinical use. The adequate osteoconductive and anti-S. aureus effects of a collagen functionalized poly(D, L-lactic acid) (PDLLA) porous scaffold loaded with minocycline (a tetracycline antibiotic) have been previously demonstrated3. In the present study, we focus on the problem of mixed bacterial-fungal biofilm infections and the joining of two antimicrobials in the PDLLA scaffold. Minocycline and voriconazole (an antifungal triazole) were the chosen model drugs, since minocycline may represent a promising drug that can be administered in combination with azoles (namely voriconazole) to treat infections caused by pathogenic Candida species. Morphological and chemical properties of the co-delivery PDLLA scaffolds, as well as drug release profiles, were examined. The antibiofilm activity of these drug delivery systems was tested against single- and dual-species biofilms of S. aureus and C. albicans. The formation of dual-species S. aureus – C. albicans biofilms was studied over time to understand the relationship between both microorganisms during in vitro biofilm formation. Cytocompatibility and osteoconductive tests were also conducted using MG-63 osteoblasts to assess the biocompatibility of the PDLLA scaffolds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationZegre M, Barros J, Ribeiro IA, Santos C, Caetano LA, Gonçalves L, et al. Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. Aureus-C. Albicans biofilms. Acta Farm Port. 2023;(Suppl.):29-32.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/16966
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOrdem dos Farmacêuuticospt_PT
dc.relation.publisherversionhttps://actafarmaceuticaportuguesa.com/index.php/afp/issue/view/30pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBone infectionpt_PT
dc.subjectMinocyclinept_PT
dc.subjectVoriconazolept_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020pt_PT
dc.titlePoly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilmspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage32pt_PT
oaire.citation.issueSupplpt_PT
oaire.citation.startPage29pt_PT
oaire.citation.titleActa Farmacêutica Portuguesapt_PT
person.familyNameAranha Caetano
person.givenNameLiliana
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.orcid0000-0003-1496-2609
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication6517c656-f913-4f54-8682-77c2856c9e4c
relation.isAuthorOfPublication.latestForDiscovery6517c656-f913-4f54-8682-77c2856c9e4c

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