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DRhoGEF2 regulates cellular tension and cell pulsations in the Amnioserosa during Drosophila dorsal closure

2011Journal article Open access
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dc.contributor.authorAzevedo, Dulce
dc.contributor.authorAntunes, Marco
dc.contributor.authorPrag, Soren
dc.contributor.authorMa, Xiaoyan
dc.contributor.authorHacker, Udo
dc.contributor.authorBrodland, G. Wayne
dc.contributor.authorHutson, M. Shane
dc.contributor.authorSolon, Jerome
dc.contributor.authorJacinto, António
dc.date.accessioned2012-08-14T14:35:16Z
dc.date.available2012-08-14T14:35:16Z
dc.date.issued2011
dc.description.abstractCoordination of apical constriction in epithelial sheets is a fundamental process during embryogenesis. Here, we show that DRhoGEF2 is a key regulator of apical pulsation and constriction of amnioserosal cells during Drosophila dorsal closure. Amnioserosal cells mutant for DRhoGEF2 exhibit a consistent decrease in amnioserosa pulsations whereas overexpression of DRhoGEF2 in this tissue leads to an increase in the contraction time of pulsations. We probed the physical properties of the amnioserosa to show that the average tension in DRhoGEF2 mutant cells is lower than wild-type and that overexpression of DRhoGEF2 results in a tissue that is more solid-like than wild-type. We also observe that in the DRhoGEF2 overexpressing cells there is a dramatic increase of apical actomyosin coalescence that can contribute to the generation of more contractile forces, leading to amnioserosal cells with smaller apical surface than wild-type. Conversely, in DRhoGEF2 mutants, the apical actomyosin coalescence is impaired. These results identify DRhoGEF2 as an upstream regulator of the actomyosin contractile machinery that drives amnioserosa cells pulsations and apical constriction.por
dc.identifier.citationAzevedo D, Antunes M, Prag S, Ma X, Hacker U, Brodland GW, et al. DRhoGEF2 regulates cellular tension and cell pulsations in the Amnioserosa during Drosophila dorsal closure. PLoS ONE. 2011;6(9):e23964.por
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.21/1722
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPLoSpor
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023964por
dc.subjectDRhoGEF2por
dc.titleDRhoGEF2 regulates cellular tension and cell pulsations in the Amnioserosa during Drosophila dorsal closurepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPagee23964por
oaire.citation.titlePLoS ONEpor
oaire.citation.volume6por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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