Repository logo
 
Publication

Electrostatic dust cloth to assess occupational exposure to bioburden in primary health care centers

2019-06Conference object Open access
Simple item page
dc.contributor.authorViegas, Carla
dc.contributor.authorSantos, Patrícia
dc.contributor.authorAlmeida, Beatriz
dc.contributor.authorMonteiro, Ana
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorViegas, Susana
dc.date.accessioned2019-07-23T10:32:55Z
dc.date.available2019-07-23T10:32:55Z
dc.date.issued2019-06
dc.descriptionProject EXPOsE, nº 23222 (02/SAICT/2016).pt_PT
dc.description.abstractPrimary health care centres (PHCC) in Portugal ensure the prevision of primary health care to the population of a specific geographic area and have a crucial role in health promotion and disease prevention. Surprisingly, there is a gap of information regarding this occupational environment, although bioburden (comprising fungi and bacteria burden) assessment and control in health care facilities is currently a mandatory and vital part of prevention strategies against hospital-acquired infections. A total of 10 primary health care canters located in the city of Lisbon were investigated for the first time. The sample collection was conducted in workstations where was consider a critical risk of occupational exposure to bioburden and active and passive sampling methods were applied. Bioburden collected by the 81 electrostatic dust cloth (EDC) was analyzed by culture based-methods and molecular detection using qPCR targeting 5 different fungi, namely 4 Aspergillus sections (Flavi, Fumigati, Circumdati and Versicolores) and Stachybotrys chartarum complex will be performed shortly. EDC weight, extraction and bioburden characterization by culture based-methods followed the same procedures already described elsewhere. Additionally, particles measurements were performed as in the same previous study. The fungal contamination levels ranged from 0 to 53397.03 CFU.m-2 (workers canteen). Among Aspergillus genera observed, the most prevalent was Aspergillus section Versicolores (0.09%) followed by Nigri (0.03%). Sections Candidi and Fumigati were also isolated in smaller counts (0.01%). The total bacterial contamination ranged from 0 CFU.m-2 to 4670.91 CFU.m-2 (treatment room). A load of Gram-negative bacteria ranged from 0 to 530.79 CFU.m-2. Among other results, statistical correlation analyses suggest that higher EDC weights are related to higher concentrations of the particles 5.0 and higher values of TPM. It was possible to identify the PHCC with higher counts regarding fungal counts (significant differences) through EDC, enabling the decision about the further need to apply different sampling approaches and also to identify potential indoor contamination sources. Thus, EDC can be applied as a screening-method for particles assessment and as a complementary method for assessing bioburden, since it provides a long-term integrated sample, whereas air samples can only reflect the load from a shorter period of time.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Santos P, Almeida B, Monteiro A, Carolino E, Viegas S. Electrostatic dust cloth to assess occupational exposure to bioburden in primary health care centers. In: INRS – Biological Risks, Nancy (France), June 5-7, 2019.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/10359
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationProject nº 23222 (02/SAICT/2016)pt_PT
dc.relationProject EXPOsEpt_PT
dc.relation.publisherversionhttps://www.inrs-risquesbiologiques2019.fr/docs/programme_inrsrb2019.en.pdf?v=20190620pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectPrimary health care centerpt_PT
dc.subjectBioburdenpt_PT
dc.subjectElectrostatic dust clothpt_PT
dc.subjectPortugalpt_PT
dc.subjectProject nº 23222 (02/SAICT/2016)pt_PT
dc.subjectProject EXPOsEpt_PT
dc.titleElectrostatic dust cloth to assess occupational exposure to bioburden in primary health care centerspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceNancypt_PT
person.familyNameViegas
person.familyNameCarolino
person.familyNameViegas
person.givenNameCarla
person.givenNameElisabete
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Electrostatic dust cloth to assess occupational exposure to bioburden in primary health care centers.pdf
Size:
91.58 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESTeSL - Posters