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Genetic diversity and drug resistance of HIV-1 among infected pregnant women newly diagnosed in Luanda, Angola

2019-11Journal article Open access
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dc.contributor.authorSebastião, Cruz S.
dc.contributor.authorNeto, Zoraima
dc.contributor.authorJesus, Carlos S. de
dc.contributor.authorMirandela, Marinela
dc.contributor.authorJandondo, Domingos
dc.contributor.authorCouto-Fernandez, José C.
dc.contributor.authorTanuri, Amilcar
dc.contributor.authorMorais, Joana
dc.contributor.authorBrito, Miguel
dc.date.accessioned2019-11-27T12:42:08Z
dc.date.available2019-11-27T12:42:08Z
dc.date.issued2019-11
dc.descriptionProject Pró-África CNPq n˚ 440145/2015-5pt_PT
dc.description.abstractMonitoring genetic diversity and drug resistance mutations (DRMs) is critical for understanding HIV epidemiology. Here, we report HIV-1 genetic diversity and DRMs in blood samples from 42 HIV-positive pregnant women naive to antiretroviral therapy (ART), in Luanda. The samples were subjected to nested-PCR, followed by sequencing of the HIV-1 pol gene, targeting the protease and reverse transcriptase fragments. HIV-1 diversity was analyzed using the REGA HIV-1 subtyping tool and DRMs were identified using the Calibrated Population Resistance tool. A total of 34 sequences were obtained. The data revealed wide HIV-1 subtypes heterogeneity, with subtype C (38%, 13/34) the most frequent, followed by the subtypes F1 (18%, 6/34), A1 (9%, 3/34), G (9%, 3/34), D (6%, 2/34) and H (3%, 1/34). In addition, recombinants strains were detected, with CRF02_AG (6%, 2/34) the most frequent, followed by CRF37_cpx, F1/C, A1/G, and H/G, all with 3% (1/34). A total of 6/34 (18%) of the sequences presented DRMs. The non-nucleoside reverse transcriptase inhibitors presented 15% (5/34) of resistance. Moreover, 1/34 (3%) sequence presented resistance against both non-nucleoside reverse transcriptase inhibitors and nucleoside reverse transcriptase inhibitors, simultaneously. Despite the small sample size, our results suggest the need to update currently used ART regimens. Surveillance of HIV-1 subtypes and DRMs are necessary to understand HIV epidemiology and to guide modification of ART guidelines in Angola.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSebastião CS, Neto Z, Jesus CS, Mirandela M, Jandondo D, Brito M, et al. Genetic diversity and drug resistance of HIV-1 among infected pregnant women newly diagnosed in Luanda, Angola. PLoS One. 2019;14(11):e0225251.pt_PT
dc.identifier.doi10.1371/journal.pone.0225251pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/10757
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relationProject Pró-África CNPq n˚ 440145/2015-5pt_PT
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225251pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectHIV infectionpt_PT
dc.subjectPregnancypt_PT
dc.subjectAntiretroviral therapypt_PT
dc.subjectAntimicrobial resistancept_PT
dc.subjectEpidemiologypt_PT
dc.subjectHuman geneticspt_PT
dc.subjectAngolapt_PT
dc.subjectLuandapt_PT
dc.subjectProject Pró-África CNPq n˚ 440145/2015-5pt_PT
dc.titleGenetic diversity and drug resistance of HIV-1 among infected pregnant women newly diagnosed in Luanda, Angolapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue11pt_PT
oaire.citation.startPagee0225251pt_PT
oaire.citation.titlePLOS ONEpt_PT
oaire.citation.volume14pt_PT
person.familyNameBrito
person.givenNameMiguel
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublication.latestForDiscovery4252d8e0-800c-4d67-8b13-0b711d860669

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