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Trends on Aspergillus epidemiology: perspectives from a national reference laboratory surveillance program

2021-01Journal article Open access
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dc.contributor.authorSabino, Raquel
dc.contributor.authorGonçalves, Paulo
dc.contributor.authorMartins-Melo, Aryse
dc.contributor.authorSimões, Daniela
dc.contributor.authorOliveira, Mariana
dc.contributor.authorFrancisco, Mariana
dc.contributor.authorViegas, Carla
dc.contributor.authorCarvalho, Dinah
dc.contributor.authorMartins, Carlos
dc.contributor.authorFerreira, Teresa
dc.contributor.authorToscano, Cristina
dc.contributor.authorSimões, Helena
dc.contributor.authorVeríssimo, Cristina
dc.date.accessioned2021-01-06T17:40:36Z
dc.date.available2021-01-06T17:40:36Z
dc.date.issued2021-01
dc.descriptionProject EXPOsE. Project nº 23222 (02/SAICT/2016).pt_PT
dc.descriptionProject Waste FRPD. Project IPL/2018/WasteFRPD_ESTeSL.pt_PT
dc.description.abstractIdentification of Aspergillus to species level is important since sibling species may display variable susceptibilities to multiple antifungal drugs and also because correct identification contributes to improving the knowledge of epidemiological studies. Two retrospective laboratory studies were conducted on Aspergillus surveillance at the Portuguese National Mycology Reference Laboratory. The first, covering the period 2017–2018, aimed to study the molecular epidemiology of 256 Aspergillus isolates obtained from patients with respiratory, subcutaneous, or systemic infections and from environmental samples. The second, using our entire collection of clinical and environmental A. fumigatus isolates (N = 337), collected between 2012 and 2019, aimed to determine the frequency of azole-resistant A. fumigatus isolates. Aspergillus fumigatus sensu stricto was the most frequent species in both clinical and environmental samples. Overall, and considering all Aspergillus sections identified, a high frequency of cryptic species was detected, based on beta-tubulin or calmodulin sequencing (37% in clinical and 51% in environmental isolates). Regarding all Fumigati isolates recovered from 2012–2019, the frequency of cryptic species was 5.3% (18/337), with the identification of A. felis (complex), A. lentulus, A. udagawae, A. hiratsukae, and A. oerlinghauensis. To determine the frequency of azole resistance of A. fumigatus, isolates were screened for azole resistance using azole-agars, and 53 possible resistant isolates were tested by the CLSI microdilution reference method. Nine A. fumigatus sensu stricto and six Fumigati cryptic isolates showed high minimal inhibitory concentrations to itraconazole, voriconazole, and/or posaconazole. Real-time PCR to detect cyp51A mutations and sequencing of the cyp51A gene and its promoter were performed. The overall frequency of resistance to azoles in A. fumigatus sensu stricto was 3.0%. With this retrospective analysis, we were able to detect one azole-resistant G54R mutant A. fumigatus environmental isolate, collected in 2015. The TR34/L98H mutation, linked to the environmental transmission route of azole resistance, was the most frequently detected mutation (N = 4; 1.4%). Our findings underline the demand for correct identification and susceptibility testing of Aspergillus isolates.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSabino R, Gonçalves P, Martins Melo A, Simões D, Oliveira M, Viegas C, et al. Trends on Aspergillus epidemiology: perspectives from a national reference laboratory surveillance program. J Fungi. 2021;7(1):28.pt_PT
dc.identifier.doi10.3390/jof7010028pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/12581
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationProject EXPOsEpt_PT
dc.relationProject nº 23222 (02/SAICT/2016)pt_PT
dc.relationProject Waste FRPDpt_PT
dc.relationProject IPL/2018/WasteFRPD_ESTeSLpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2309-608X/7/1/28pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAspergilluspt_PT
dc.subjectSurveillancept_PT
dc.subjectMolecular epidemiologypt_PT
dc.subjectCryptic speciespt_PT
dc.subjectAzole resistance mutationspt_PT
dc.subjectProject EXPOsEpt_PT
dc.subjectProject nº 23222 (02/SAICT/2016)pt_PT
dc.subjectProject Waste FRPDpt_PT
dc.subjectProject IPL/2018/WasteFRPD_ESTeSLpt_PT
dc.titleTrends on Aspergillus epidemiology: perspectives from a national reference laboratory surveillance programpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage28pt_PT
oaire.citation.titleJournal of Fungipt_PT
oaire.citation.volume7pt_PT
person.familyNameViegas
person.givenNameCarla
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.orcid0000-0002-1545-6479
person.identifier.ridB-7217-2013
person.identifier.scopus-author-id55443609700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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