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Sampling methods and assays applied in SARS-CoV-2 exposure assessment

2021-06Journal article Open access
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dc.contributor.authorPena, Pedro
dc.contributor.authorMorais, Joana
dc.contributor.authorGomes, Anita Q.
dc.contributor.authorViegas, Carla
dc.date.accessioned2021-02-24T10:54:05Z
dc.date.available2024-02-24T01:30:20Z
dc.date.issued2021-06
dc.description.abstractThe SARS-CoV-2 exposure assessment is critical to implement control measures and guarantee the safety of patients and workers from different occupational environments. The aim of this review article was to identify methodologies applied for SARS-CoV-2 sampling and analyses in environmental samples in different occupational and indoor environments. This study reports the search of available data published between May 29th, 2020, and November 1st, 2020. The search strategy used allowed the identification of 48 papers that comply with selected inclusion and exclusion criteria. The most described indoor environment consisted of health care facilities. From all the analyzed studies, 34 sampled surfaces, 27 sampled air (impactors and impingers being the most used), and 9 sampled water. All studies were based on molecular detection by qPCR of viral RNA extracted from collected samples. SARS-CoV-2 was detected in 44 out of the 48 studies. The results suggest that the sampling approach should include both active and passive sampling methods in order to overcome each method's limitations. Concerning the assays used, although most studies were based on qPCR detection, the fact that the digital PCR technique allows SARS-CoV-2 detection at lower concentrations indicates that this should be the chosen method for future detection studies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPena P, Morais J, Gomes AQ, Viegas C. Sampling methods and assays applied in SARS-CoV-2 exposure assessment. Sci Total Environ. 2021;775:145903.pt_PT
dc.identifier.doi10.1016/j.scitotenv.2021.145903pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/12935
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0048969721009700pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectExposure assessmentpt_PT
dc.subjectSARS-CoV-2pt_PT
dc.subjectIndoor airpt_PT
dc.subjectSamplingpt_PT
dc.subjectAssayspt_PT
dc.titleSampling methods and assays applied in SARS-CoV-2 exposure assessmentpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage145903pt_PT
oaire.citation.titleScience of The Total Environmentpt_PT
oaire.citation.volume775pt_PT
person.familyNameGomes
person.familyNameViegas
person.givenNameAnita
person.givenNameCarla
person.identifier.ciencia-id4B10-E015-52B7
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.orcid0000-0002-3348-0448
person.identifier.orcid0000-0002-1545-6479
person.identifier.ridC-3580-2014
person.identifier.ridB-7217-2013
person.identifier.scopus-author-id7202386033
person.identifier.scopus-author-id55443609700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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