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A Flow Injection Methodology for Acetamide Determination Using a Tubular Bioreactor and na Ammonium Sensor †

dc.contributor.authorVeríssimo, Marta I. S.
dc.contributor.authorSilva, Nelson A. F.
dc.contributor.authorKarmali, Amin
dc.contributor.authorGomes, Maria Teresa S. R.
dc.date.accessioned2018-01-03T10:32:35Z
dc.date.available2018-01-03T10:32:35Z
dc.date.issued2017-12-04
dc.description.abstractPseudomonas aeruginosa (P. aeruginosa) is a Gram-negative bacterium quite versatile that grows in the soil, in coastal marine habitats, as well as in the tissues of plants and animals. P. aeruginosa is the source of amidase (acylamide amidohydrolase E.C. 3.5.1.4) which catalyzes the hydrolysis of a small range of short aliphatic amides into the corresponding carboxylic acids and ammonia. A low cost piezoelectric quartz crystal coated with a selective membrane for ammonium was used to detect the reaction product. Conversion of amide into the correspondent amine was achieved both with cell-free extract of P. Aeruginosa or the whole cells. This conversion was first performed in batch and later on injected into the sensor system where a buffer carrier was flowing over the coated crystal. Another approach consisted in incorporating a conversion reactor with the immobilized cell-free extract of P. Aeruginosa in the FIA system. Amide solutions were injected and carried by the buffer stream through the reactor and then directed to the sensor. Different supports were used for immobilization, such as calcium alginate beads, glass beads and the inside walls of a hollow glass column. The best arrangement allowed acetamide determination without sensitivity lost for 1-month period.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVERISSIMO, Marta I. S.; SILVA, Nelson A. F.; KARMALI, Amin; GOMES, Maria Teresa S. R. – A flow injection methodology for acetamide determination using a tubular bioreactor and na ammonium sensor †. In 5th International Symposium on Sensor Science (I3S 2017). Barcelona, Spain: MDPI, 2017. Vol. 1 (8). P. 785.pt_PT
dc.identifier.doi10.3390/proceedings1080785pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/7850
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttp://www.mdpi.com/2504-3900/1/8/785pt_PT
dc.titleA Flow Injection Methodology for Acetamide Determination Using a Tubular Bioreactor and na Ammonium Sensor †pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceBarcelona, Spainpt_PT
oaire.citation.title5th International Symposium on Sensor Science (I3S 2017)pt_PT
oaire.citation.volume1pt_PT
person.familyNameKarmali
person.givenNameAmin
person.identifier.orcid0000-0003-0419-401X
person.identifier.ridE-4787-2011
person.identifier.scopus-author-id6603778216
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublication.latestForDiscovery23975b4b-3234-4139-9675-89ea6f9a2332

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