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Authors
Abstract(s)
A investigação e o desenvolvimento da impressão tridimensional (3D) para aplicações na área da engenharia biomédica tem aumentado significativamente na última década. A impressão 3D permite criar estruturas complexas e personalizadas, adaptadas às características individuais de cada paciente, apresentando benefícios nos tratamentos de diversas condições médicas e reduzindo os custos associados. O desenvolvimento de biomateriais naturais, com propriedades biológicas melhoradas devido à sua biocompatibilidade e não toxicidade, é atualmente uma área em expansão e com um grande potencial de aplicação. Os antioxidantes são substâncias que têm demonstrado a sua capacidade de proteger contra os radicais livres e controlar o stress oxidativo das células. A combinação de biopolímeros com compostos que apresentam uma atividade antioxidante comprovada é particularmente interessante para a produção de pensos para feridas. Neste contexto, o presente trabalho teve como objetivo a produção de filmes de quitosano e alginato dopados com ácido ascórbico e quercetina, com o objetivo de produzir materiais biocompatíveis, com possíveis propriedades antioxidantes. Os filmes foram produzidos pelo método de casting e estudou-se o perfil de libertação dos compostos incorporados. De modo a avaliar a atividade antioxidante dos filmes produzidos foi utilizado o método do DPPH e os resultados demonstraram que a incorporação de quercetina permitiu obter filmes de quitosano e alginato com atividade antioxidante. Para avaliar a sua utilização em tecnologias de fabrico aditivo, este trabalho envolveu a modelação e criação de um módulo de seringa personalizado para desenvolver uma impressora 3D por extrusão por seringa, de baixo custo, adaptada ao fabrico de estruturas tridimensionais de quitosano e alginato. Foi possível produzir ambas as estruturas, no entanto as estruturas impressas de alginato demonstraram uma qualidade superior comparativamente com as de quitosano.
The research and the development of the three-dimensional (3D) printing for biomedical applications has increased significantly during the last decade. In addition, 3D printing allows the production of complex and personalized structures, adapted to the individual characteristics of each patient, presenting beneficials in the treatments of several medical conditions and reducing the related costs. The development of natural biomaterials with enhanced biological properties, due to their biocompatibility and non-toxicity, is nowadays a growing field with a vast potential. Antioxidants are substances that have shown their capacity for protection against free radicals and control of oxidative stress in cells. The combination of biopolymers with proven antioxidants compounds is particularly interesting to the production of wound dressings. In this context, the present study reports the production of chitosan and alginate films doped with ascorbic acid and quercetin, to produce biocompatible materials, with possible antioxidant properties. Films were produced by casting method and the release profile of incorporated compounds was studied. To evaluate the antioxidant activity of the produced films, DPPH standard method was used, and the results showed that the incorporation of quercetin allowed to obtain chitosan and alginate films with antioxidant activity. To evaluate its use in additive manufacturing technologies, this work involved modeling and creating a customized syringe module to develop a low-cost syringe extrusion 3D printer, adapted to the manufacture of three-dimensional chitosan and alginate structures. It was possible to produce both structures, with alginate structures achieved with higher quality than chitosan.
The research and the development of the three-dimensional (3D) printing for biomedical applications has increased significantly during the last decade. In addition, 3D printing allows the production of complex and personalized structures, adapted to the individual characteristics of each patient, presenting beneficials in the treatments of several medical conditions and reducing the related costs. The development of natural biomaterials with enhanced biological properties, due to their biocompatibility and non-toxicity, is nowadays a growing field with a vast potential. Antioxidants are substances that have shown their capacity for protection against free radicals and control of oxidative stress in cells. The combination of biopolymers with proven antioxidants compounds is particularly interesting to the production of wound dressings. In this context, the present study reports the production of chitosan and alginate films doped with ascorbic acid and quercetin, to produce biocompatible materials, with possible antioxidant properties. Films were produced by casting method and the release profile of incorporated compounds was studied. To evaluate the antioxidant activity of the produced films, DPPH standard method was used, and the results showed that the incorporation of quercetin allowed to obtain chitosan and alginate films with antioxidant activity. To evaluate its use in additive manufacturing technologies, this work involved modeling and creating a customized syringe module to develop a low-cost syringe extrusion 3D printer, adapted to the manufacture of three-dimensional chitosan and alginate structures. It was possible to produce both structures, with alginate structures achieved with higher quality than chitosan.
Description
Trabalho final de Mestrado para obtenção do grau de Mestre em Engenharia Biomédica
Keywords
Filmes biocompatíveis Atividade antioxidante Impressão 3D Estruturas tridimensionais Extrusão por seringa Biocompatible films Antioxidant activity 3D printing Three-dimensional structures
Citation
Paz, Catarina Martins- Produção de estruturas de quitosano e alginato por impressora de extrusão por seringa. Lisboa: Instituto Superior de Engenharia de Lisboa, 2023. Dissertação de Mestrado