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The comet assay as a tool in human biomonitoring studies: effects of confounding factors

2025-11Artigo científico Acesso aberto
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datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorMøller, Peter
dc.contributor.authorGajski, Goran
dc.contributor.authorGerić, Marko
dc.contributor.authorGiovannelli, Lisa
dc.contributor.authorAzqueta, Amaya
dc.contributor.authorHaveric, Anja
dc.contributor.authorStopper, Helga
dc.contributor.authorBankoglu, Ezgi Eyluel
dc.contributor.authorCollins, Andrew
dc.contributor.authorLadeira, Carina
dc.date.accessioned2025-12-15T14:52:05Z
dc.date.available2025-12-15T14:52:05Z
dc.date.issued2025-11
dc.descriptionThis work was supported by the affiliated institutions, European Regional Development Fund project KK.01.1.1.02.0007 (Rec-IMI), the Croatian Science Foundation (HUMNap project #1192), the Horizon Europe (EDIAQI project #101057497), the European Union—Next Generation EU 533–03–23–0006 (BioMolTox), FCT/MCTES UIDP/ 05608/2020 (10.54499/UIDP/05608/2020) and UIDB/05608/2020 (10.54499/UIDB/05608/2020), and the International Comet Assay Working Group (ICAWG).
dc.description.abstractThe comet assay is widely used in human biomonitoring studies of environmental and occupational exposures. However, it is clear from multiple studies that various types of confounding factors might affect the direct relationship between exposure and DNA damage in the comet assay. In addition to common confounders such as age, sex, and smoking, other factors considered to be important determinants for background levels of DNA damage in the comet assay include exhaustive physical exercise, chronic diseases, medical treatment, and diet. These are typically controlled in biomonitoring studies by restriction or matching of subjects. Period effects (or seasonal variation) have been observed in a relatively large number of studies. There are various putative factors, which may cause period effects, including temporal variation in solar radiation, temperature, and air pollution. The present review describes the effects of these confounding factors in measurements of DNA strand breaks by the comet assay. In general, the literature does not indicate that any confounding factor is consistently associated with an increased level of DNA damage, measured by the comet assay. In this respect, it is important to balance the need to control for confounding with the risk of introducing in the statistical analysis a variable, which is influenced by exposure and outcome (i.e. collider bias). In addition, it is important that investigators describe procedures for controlling the effect of confounding factors in the selection of subjects and statistical analysis. Care should be taken in study design and statistical analysis to avoid unwanted effects of confounding factors.eng
dc.identifier.citationMøller P, Gajski G, Gerić M, Giovannelli L, Azqueta A, Ladeira C, et al. The comet assay as a tool in human biomonitoring of exposure to heavy metals: effects of confounding factors. Mutat Res Rev Mutat Res. 2025;796:108566.
dc.identifier.doi10.1016/j.mrrev.2025.108566
dc.identifier.issn1383-5742
dc.identifier.urihttp://hdl.handle.net/10400.21/22314
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S1383574225000377
dc.relation.ispartofMutation Research - Reviews in Mutation Research
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBiomonitoring
dc.subjectAge
dc.subjectComet assay
dc.subjectLifestyle
dc.subjectSeasonal variation
dc.subjectSex
dc.titleThe comet assay as a tool in human biomonitoring studies: effects of confounding factorseng
dc.typejournal article
dcterms.referenceshttps://doi.org/10.1016/j.mrrev.2025.108566
dspace.entity.typePublication
oaire.citation.startPage108566
oaire.citation.titleMutation Research - Reviews in Mutation Research
oaire.citation.volume796
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLadeira
person.givenNameCarina
person.identifier144237
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.orcid0000-0001-5588-0074
person.identifier.ridJ-2572-2012
person.identifier.scopus-author-id36463788000
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

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