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Advisor(s)
Abstract(s)
Rotavirus alphagastroenteritidis (R. alphagastroenteritidis) remains the leading cause of pediatric diarrhea. Although Angola introduced Rotarix®, the human monovalent R. alphagastroenteritidis vaccine since 2014 as part of its routine childhood immunization program, no follow-up study has been conducted. This study aimed to evaluate the distribution of R. alphagastroenteritidis genotypes among children under five years of age, hospitalized with acute gastroenteritis (AGE), after the introduction of the rotavirus vaccine. To achieve this goal, stool samples collected between 2021 and 2022 from children under 5 years of age diagnosed with AGE at six hospitals in Luanda Province were analyzed. The R. alphagastroenteritidis-antigen immunochromatographic test (SD Bioline™, Abbott, Chicago, IL, USA) was performed, and 121 positive samples were genotyped. Ten samples were randomly selected for further Sanger sequencing. The results showed that the G9P[6] was the most prevalent genotype (17.3%), followed by G9P[8] (16.5%), G2P[4] (14.9%), G3P[6] (13.2%), G8P[6] (11.5%), and less frequently G12P[8] (9.1%), G1P[6] (4.1%), and G1P[8] (2.5%). The genotype combinations G3P[6], G8P[6], and G12P[8] were detected for the first time in Luanda Province. In conclusion, the emergence of new genotype combinations supports the need for continuous surveillance to identify the trend in R. alphagastroenteritidis infection and the emergence of new strains circulating in Luanda Province in the post-vaccination period.
Description
This research was funded by Abbott Rapid Diagnostics Group, the National Health Research Institute (INIS), Luanda, Angola, the Centro de Investigação em Saúde de Angola (CISA), Angola, and the Gulbenkian Foundation, Lisbon, Portugal. The study sponsors had no role in the design of this study.
Data Availability Statement: All the sequences obtained in the present work are available in the GenBank repository under accession numbers PQ139230 to PQ139236 (VP4 gene sequences) and PQ139237 to PQ139243 (VP7 gene sequences). Any remaining data are available on request from the authors.
Keywords
Rotarix Rotavirus vaccine Genotyping Rotavirus alphagastroenteritidis Angola Província de Luanda
Citation
Vita DG, Santiso-Bellón C, Lemos M, Neto Z, Fortes-Gabriel E, Brito M, et al. Rotavirus alphagastroenteritidis: circulating strains after the introduction of the Rotavirus vaccine (Rotarix®) in Luanda Province of Angola. Viruses. 2025;17(6):858.
Publisher
MDPI AG