Repository logo
 
Publication

The Tetrahymena chaperonin subunit CCT eta gene is coexpressed with CCT gamma gene during cilia biogenesis and cell sexual reproduction

1996-04Journal article Open access
Simple item page
dc.contributor.authorCyrne, Luísa
dc.contributor.authorGuerreiro, Paulo
dc.contributor.authorCardoso, Ana Cristina
dc.contributor.authorRodrigues-Pousada, Claudina
dc.contributor.authorSoares, Helena
dc.date.accessioned2014-01-17T12:24:13Z
dc.date.available2014-01-17T12:24:13Z
dc.date.issued1996-04
dc.description.abstractWe report here the cloning and the characterization of the T. pyriformis CCT eta gene (TpCCT eta) and also a partial sequence of the corresponding T. thermophila gene (TtCCT eta). The TpCCt eta gene encodes a protein sharing a 60.3% identity with the mouse CCT eta. We have studied the expression of these genes in Tetrahymena exponentially growing cells, cells regenerating their cilia for different periods and during different stages of the cell sexual reproduction. These genes have similar patterns of expression to those of the previously identified TpCCt gamma gene. Indeed, the Tetrahymena CCT eta and CCT gamma genes are up-regulated at 60-120 min of cilia recovery, and in conjugation when vegetative growth was resumed and cell division took place. Our results seem to indicate that both CCT subunits play an important role in the biogenesis of the newly synthesized cilia of Tetrahymena and during its cell division.por
dc.identifier.citationCyrne L, Guerreiro P, Cardoso AC, Rodrigues-Pousada C, Soares H. The Tetrahymena chaperonin subunit CCT eta gene is coexpressed with CCT gamma gene during cilia biogenesis and cell sexual reproduction. FEBS Lett. 1996;383(3):277-83.por
dc.identifier.issn1873-3468
dc.identifier.urihttp://hdl.handle.net/10400.21/3114
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/0014579396002402#por
dc.subjectCCTη-chaperonin genepor
dc.subjectCiliated protozoanpor
dc.subjectGene structurepor
dc.subjectGene expressionpor
dc.subjectChaperonin containing TCP-1por
dc.subjectCloning, Molecularpor
dc.subjectDNA primerspor
dc.subjectGenes, Protozoanpor
dc.subjectMacromolecular substancespor
dc.subjectMolecular sequence datapor
dc.subjectProtozoan proteinspor
dc.subjectRecombinant proteinspor
dc.subjectSequence homology, Amino acidpor
dc.subjectTetrahymena pyriformispor
dc.titleThe Tetrahymena chaperonin subunit CCT eta gene is coexpressed with CCT gamma gene during cilia biogenesis and cell sexual reproductionpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage283por
oaire.citation.startPage277por
oaire.citation.titleFEBS Letterspor
oaire.citation.volume383por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
The Tetrahymena chaperonin subunit CCTrl gene.pdf
Size:
707.59 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESTeSL - Artigos