Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII

Guerreiro, Paulo; Azevedo, Dulce; Barreiros, Tânia; Rodrigues-Pousada, Claudina

http://hdl.handle.net/10400.21/3088

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Authors

Guerreiro, Paulo

Azevedo, Dulce

Barreiros, Tânia

Rodrigues-Pousada, Claudina

Abstract(s)

A 9.9 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains four open reading frames (ORFs) longer than 100 amino acids. One gene, PFK1, has already been cloned and sequenced and the other one is the probable yeast gene coding for the beta-subunit of the succinyl-CoA synthetase. The two remaining ORFs share homology with the deduced amino acid sequence (and their physical arrangement is similar to that) of the YHR161c and YHR162w ORFs from chromosome VIII.

Keywords

Saccharomyces cerevisiae Chromosome VII Chromosome VIII PFK1 O44 protein Amino acid sequence Chromosome mapping Chromosomes, Fungal DNA Genome, Fungal Molecular sequence data Open reading frames Plasmids Restriction mapping Sequence alignment Succinate-CoA ligases

URI

http://hdl.handle.net/10400.21/3088

Citation

Guerreiro P, Azevedo D, Barreiros T, Rodrigues-Pousada C. Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII. Yeast. 1997;13(3):275-80.