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Molecular characterization of Giardia lamblia in children less than 5 years of age with diarrhoea attending the Bengo General Hospital, Angola

2017-11Journal article Open access
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dc.contributor.authorGasparinho, Carolina
dc.contributor.authorFerreira, Filipa S.
dc.contributor.authorMayer, António Carlos
dc.contributor.authorMirante, Maria Clara
dc.contributor.authorVaz Nery, Susana
dc.contributor.authorSantos-Reis, Ana
dc.contributor.authorPortugal-Calisto, Daniela
dc.contributor.authorBrito, Miguel
dc.date.accessioned2018-03-01T14:36:54Z
dc.date.available2018-03-01T14:36:54Z
dc.date.issued2017-11
dc.description.abstractIntroduction - Giardia lamblia is a pathogenic intestinal protozoan with high prevalence in developing countries, especially among children. Molecular characterization has revealed the existence of eight assemblages, with A and B being more commonly described in human infections. Despite its importance, to our knowledge, this is the first published molecular analysis of G. lamblia assemblages in Angola. Methods - The present study aimed to identify the assemblages of G. lamblia in children with acute diarrhoea presenting at the Bengo General Hospital, Angola. A stool sample was collected and microscopy and immunochromatographic tests were used. DNA was extracted and assemblage determination was performed through amplification of the gene fragment ssu-rRNA (175 bp) and β-giardin (511 bp) through polymerase chain reaction and DNA sequencing. Results - Of the 16 stool samples screened, 12 were successfully sequenced. Eleven isolates were assigned to assemblage B and one to assemblage A. Subassemblage determination was not possible for assemblage B, while the single isolate assigned to assemblage A was identified as belonging to subassemblage A3. Conclusion - This study provides information about G. lamblia assemblages in Bengo Province, Angola and may contribute as a first step in understanding the molecular epidemiology of this protozoan in the country. GenBank accession numbers for the ssur-RNA gene: MF479750, MF479751, MF479752, MF479753, MF479754, MF479755, MF479756, MF479757, MF479758, MF479759, MF479760, MF479761. GenBank accession numbers for the β-giardin gene: MF565378, MF565379, MF565380, MF565381.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGasparinho C, Ferreira FS, Mayer AC, Mirante MC, Vaz Nery S, Brito M, et al. Molecular characterization of Giardia lamblia in children less than 5 years of age with diarrhoea attending the Bengo General Hospital, Angola. Trans R Soc Trop Med Hyg. 2017;111(11):497-503.pt_PT
dc.identifier.doi10.1093/trstmh/try004pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/8178
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relation.publisherversionhttps://academic.oup.com/trstmh/article/111/11/497/4846350pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectGenotypingpt_PT
dc.subjectGiardia lambliapt_PT
dc.subjectChildrenpt_PT
dc.subjectDiarrhoeapt_PT
dc.subjectHospitalpt_PT
dc.subjectAngolapt_PT
dc.subjectProvíncia do Bengopt_PT
dc.titleMolecular characterization of Giardia lamblia in children less than 5 years of age with diarrhoea attending the Bengo General Hospital, Angolapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage503pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage497pt_PT
oaire.citation.titleTransactions of The Royal Society of Tropical Medicine and Hygienept_PT
oaire.citation.volume111pt_PT
person.familyNameBrito
person.givenNameMiguel
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublication.latestForDiscovery4252d8e0-800c-4d67-8b13-0b711d860669

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