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- Assessing T-lymphocyte activation by non-conventional metabolomicsPublication . Ramalhete, Luís; Calado, Cecília; Sancho, M. RosárioThe current methodologies to evaluate T-cell function are based on very laborious or/and expensive methods. Nevertheless, the assessment of the appropriate functioning of these cells is very important in the clinical context, namely as a diagnostic tool of T-cell alloreactivity in different hematological settings, solid organ transplantation and immunosuppressive therapy. In the present work, we evaluated the potential of Non-conventional Metabolomics (NM) based on FTIR spectroscopy, in the assessment of T lymphocyte function after mitogen activation by phytohemagglutinin (PHa).
- Impact of the human mesenchymal stem cells donor on conditional medium compositionPublication . Pereira, Maria João Canha; Ramalhete, Luís; Aleixo, Sandra; da Silva, Cláudia L.; Cabral, Joaquim M.S.; Calado, Cecília; Fernandes, AnaExosomes produced by Mesenchymal Stem Cells (MSCs) can represent a very appealing strategy for cell-free therapies. However, to achieve this reality it is necessary to further understand the process associated to the MSC culture when conditioned to produce exosomes. In the present work, it was evaluated how different MSC obtained from different donors may affect the conditioned media composition and how this can be influenced by the conditioned media type (DMEM versus Xeno-Free medium, XF). The molecular fingerprint of the conditioned media composition was obtained by mid-infrared (MIR) spectroscopy, as this technique reflects fundamental vibrations of a high diversity of functional chemical groups present in biological samples. It was observed by principal component analysis of the second derivative spectra of conditioned media that the media chemical composition depends more from the MSCs donor than the conditioning days. Diverse spectral regions, characteristic of defined chemical groups, enabled to discriminate the chemical composition of the media according to the MSC donor. All of this was observed in both types of media (DMEM and XF). This work is a step forward to understand how different MSC donors and conditioned media may affect the exosomes characteristics.