Percorrer por autor "Sushchenko, Yelyzaveta"
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- Antibody production for diagnostic applications and therapeutic development: is it possible to reduce the use of animals?Publication . Sushchenko, Yelyzaveta; Turiel, Ema; Aguiar, Joana; Janeiro, Mariana; Borges-Ferro, ATraditionally, antibodies have been produced using the hybridoma technique, which involves immunization and the sacrifice of animals. Although this has revolutionized the practice of “traditional” immunohistochemistry, it currently raises some ethical questions regarding animal suffering. Therefore, it is important to develop methods that are viable to obtain quality antibodies for human diagnosis and therapy, while also having a less significant impact on animal welfare. There are several promising methods, such as the use of mammalian cells, the expression of antibodies in phage display, and even in yeasts. This review aims to explore alternative methods for antibody production, highlighting their advantages and limitations.