Browsing by Author "Sousa, Margarida Rodriguez"
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- Internship: exposure assessment of microbial contamination in different indoor environmentsPublication . Sousa, Margarida Rodriguez; Viegas, CarlaABSTRACT: This internship report is part of the Project/Thesis/Internship course in the 2nd year of the Master's program in Clinical Laboratory Technologies at the Lisbon School of Health Technology (ESTeSL). The professional internship took place at ESTeSL's Environmental Health Laboratory from September 2023 to May 2024, focusing on Public Health and Occupational Exposure. During the internship, sampling campaigns were conducted in primary schools and sawmills to evaluate microbiological contamination (bacteria and fungi) by collecting bioaerosols, using active and passive methods. In the laboratory, activities encompassed classical microbiology (cultureomics) and molecular techniques such as qPCR and DNA extraction. Additionally, an SOP was developed for screening azoles in Aspergillus section Fumigati isolates to define the resistance profile, and a systematic review on WWTP was authored, alongside participation in a congress. These tasks facilitated the consolidation of theoretical knowledge from the 1st year of the Master's program and the acquisition of competencies in Occupational Health, Microbiology, and Molecular Techniques, achieving the programmed objectives. This enriching experience also provided valuable skills in research and the daily life of a researcher, contributing significantly to future professional development.