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Browsing by Author "Pereira, Cristiana Costa"

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  • DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
    Publication . Bonassi, Stefano; Ceppi, Marcello; Møller, Peter; Azqueta, Amaya; Milić, Mirta; Monica, Neri; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine A. S.; Teixeira, João Paulo; Bruzzone, Marco; Da Silva, Juliana; Benedetti, Danieli; Cavallo, Delia; Ursini, Cinzia Lucia; Giovannelli, Lisa; Moretti, Silvia; Riso, Patrizia; Del Bo’, Cristian; Russo, Patrizia; Dobrzyńska, Malgorzata; Goroshinskaya, Irina A.; Surikova, Ekaterina I.; Staruchova, Marta; Barančokova, Magdalena; Volkovova, Katarina; Kažimirova, Alena; Smolkova, Bozena; Laffon, Blanca; Valdiglesias, Vanessa; Pastor, Susana; Marcos, Ricard; Hernández, Alba; Gajski, Goran; Spremo-Potparević, Biljana; Živković, Lada; Boutet-Robinet, Elisa; Perdry, Hervé; Lebailly, Pierre; Perez, Carlos L.; Basaran, Nursen; Nemeth, Zsuzsanna; Safar, Anna; Dusinska, Maria; Collins, Andrew; Anderson, Diana; Andrade, Vanessa; Pereira, Cristiana Costa; Costa, Solange; Gutzkow, Kristine B.; Ladeira, Carina; Moretti, Massimo; Costa, Carla; Orlow, Irene; Rojas, Emilio; Pourrut, Bertrand; Kruszewski, Marcin; Knasmueller, Siegfried; Shaposhnikov, Sergey; Žegura, Bojana; Stopper, Helga
    The comet assay or single cell gel electrophoresis is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated with DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modeled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
    2021-08Journal article Open access Show more
  • Endotoxin levels in forklift’s filters and the importance of maintenance enforcement
    Publication . Pereira, Cristiana Costa; Lage, Bruna; Teixeira, João Paulo; Viegas, Carla
    Many studies have already linked endotoxin exposure, especially in workplaces, to airways disease, yet threshold limit values have not been set, nor a standardized procedure for the analysis of endotoxin levels. Inhaled endotoxins may cause bronchial asthma and may increase the allergic response in hypersensitive people. Endotoxins are biologically active lipopolysaccharides (LPSs) produced by the most external layer of cellular walls of gram (−) bacteria. They are commonly found at workplaces where large amounts of bioaerosols are generated, like in waste sorting plants, where microorganisms growing on organic residue are present at high concentration in the air. Endotoxins are relatively heated stable so they are released into the air during cell growth and after cells’ death when the integrity of the cell wall is ruptured. Up to the present, endotoxin values are measured with non-uniform methods and therefore values are of limited benefit for assessment and limits’ establishment. In this study, eleven filters belonging to the filtration system from forklifts operating in one waste sorting industry located in the Lisbon region and one control filter were analyzed.
    2018-10Conference object Open access Show more