Percorrer por autor "Nunes, Catarina dos Santos Marques"
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- Global diversity, phylogenetic analysis and molecular detection of blaKPC and blaOXA genesPublication . Nunes, Catarina dos Santos Marques; Perdigão, João Ruben Mota; Gomes, Anita QuintalABSTRACT - Bacterial infections are a worldwide concern. Strains responsible for these infections, such as carbapenem-resistant-Enterobacterales, have acquired a wide variety of carbapenemases becoming resistant to a large range of carbapenems, especially Klebsiella pneumoniae strains. In these species, the prominent genes coding for carbapenemases appear to be blaKPC and blaOXA. As a result, this project focused on global diversity and phylogenetic analyses, as well as on the molecular detection of these two genes. For phylogenetic diversity analyses, we obtained 88 blaKPC and 428 blaOXA reference sequences from the NCBI. These sequences were then used in a programmatic tblastn against the entire nucleotide database at NCBI for taxonomic dispersion analyses, yielding 1232 blaKPC and 2204 blaOXA alleles. Three KPC groups and five OXA groups could thus be established. Furthermore, we discovered three Tn4401 isoforms (Tn4401a, Tn4401b, and Tn4401d) linked to 450 blaKPC, as well as a blaKPC-31 gene found in a Tn4401d. A blaOXA-51 gene was discovered in Salmonella and five blaOXA-58 genes in Proteus. Furthermore, using herein-designed oligonucleotides, blaKPC or blaOXA genes were successfully amplified from 52 Enterobacterales isolates. Also, no correlation was found between the carriage of blaKPC-181 and altered susceptibility to ceftazidime-avibactam. Overall, we were able to establish previously unreported KPC groups, identify a blaKPC-31 gene within a Tn4401d, and the presence of a blaOXA-51 gene in Salmonella. Additionally, a specific multiplex PCR detection method was designed and implemented for blaKPC and blaOXA-48-like while also establishing no correlation between the emergence of blaOXA-181 and decreased susceptibility to ceftazidime-avibactam.