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Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways

2004-04-08Journal article Restricted access
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dc.contributor.authorFerreira, Bruno S.
dc.contributor.authorCalado, Cecília
dc.contributor.authorKeulen, Frederikvan
dc.contributor.authorFonseca, Luís P. P.
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorR., Da Fonseca M. M.
dc.date.accessioned2020-09-30T12:20:33Z
dc.date.available2020-09-30T12:20:33Z
dc.date.issued2004-04-08
dc.description.abstractAlthough the metabolism and physiology of the growth of yeast strains has been extensively studied, many questions remain unanswered when the induced production of a recombinant protein is concerned. This work addresses the production of a Fusarium solani pisi cutinase by a recombinant Saccharomyces cerevisiae strain induced through the use of a galactose promoter. It was observed that whenever the strain needed to activate biosynthetic pathways, either for cutinase synthesis, or for the synthesis of the enzymes required for galactose intake, acetate production occurred. The on-line detection of acetate in the medium might prove useful for the control and the supervision of recombinant protein production processes using yeast. The volumes of acid and base added to control the pH throughout the time course of the cultivations were used to calculate an on-line estimator for acetate concentration.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFERREIRA, Bruno S.; [et al] – Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways. Journal of Biotechnology. ISSN 0168-1656. Vol. 109, N.º 1-2 (2004), pp. 159-167pt_PT
dc.identifier.doi10.1016/j.jbiotec.2003.10.033pt_PT
dc.identifier.issn0168-1656
dc.identifier.urihttp://hdl.handle.net/10400.21/12265
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPRAXIS XXI programme (project 2/2.1/BIO/1061/95, grant GGPXXI/BD/2936/96) - FCTpt_PT
dc.relationGrant GGP XXI/BD/18276/98 - FCTpt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectHeterologous cutinasept_PT
dc.subjectPhysiologypt_PT
dc.subjectAcetatept_PT
dc.titleRecombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathwayspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage167pt_PT
oaire.citation.issue1-2pt_PT
oaire.citation.startPage159pt_PT
oaire.citation.titleJournal of Biotechnologypt_PT
oaire.citation.volume109pt_PT
person.familyNameCalado
person.familyNameP. Fonseca
person.familyNameCabral
person.familyNameda Fonseca
person.givenNameCecília
person.givenNameLuis
person.givenNameJoaquim M.S.
person.givenNameMaria Manuela
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-id951D-DF38-3397
person.identifier.ciencia-idAB13-9ED3-C821
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0001-8429-6977
person.identifier.orcid0000-0002-2405-5845
person.identifier.orcid0000-0002-1498-6195
person.identifier.ridE-2102-2014
person.identifier.ridA-4228-2013
person.identifier.ridG-2052-2010
person.identifier.ridI-1321-2012
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id55916288400
person.identifier.scopus-author-id7201350203
person.identifier.scopus-author-id7005461168
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication5c533551-5113-4c0a-93f1-9c50cbd796bc
relation.isAuthorOfPublication596986f6-6f41-4107-a097-bc205ecdb95a
relation.isAuthorOfPublication9bfc3a59-5577-4b4b-aaa0-7875d468d981
relation.isAuthorOfPublication.latestForDiscovery9bfc3a59-5577-4b4b-aaa0-7875d468d981

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