Repository logo
 
Publication

Antibiotic activity screened by the rheology of S. aureus cultures

2020-06Journal article Open access
Simple item page
dc.contributor.authorPortela, Raquel
dc.contributor.authorValcovo, Filipe
dc.contributor.authorAlmeida, Pedro L.
dc.contributor.authorSobral, Rita
dc.contributor.authorLeal, Catarina R.
dc.date.accessioned2020-05-25T17:35:00Z
dc.date.available2020-05-25T17:35:00Z
dc.date.issued2020-06
dc.description.abstractMultidrug resistant bacteria are one of the most serious public health threats nowadays. How bacteria, as a population, react to the presence of antibiotics is of major importance to the outcome of the chosen treatment. In this study we addressed the impact of oxacillin, a β-lactam, the most clinically relevant class of antibiotics, in the viscosity profile of the methicillin resistant Staphylococcus aureus (MRSA) strain COL. In the first approach, the antibiotic was added, at concentrations under the minimum inhibitory concentration (sub-MIC), to the culture of S. aureus and steady-state shear flow curves were obtained for discrete time points during the bacterial growth, with and without the presence of the antibiotic, showing distinct viscosity progress over time. The different behaviors obtained led us to test the impact of the sub-inhibitory concentration and a concentration that inhibited growth. In the second approach, the viscosity growth curves were measured at a constant shear rate of 10 s−1, over time. The obtained rheological behaviors revealed distinctive characteristics associated to the presence of each concentration of the tested antibiotic. These results bring new insights to the bacteria response to a well-known bacteriolytic antibiotic.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPORTELA, Raquel; [et al] – Antibiotic activity screened by the rheology of S. aureus cultures. Fluids. ISSN 2311-5521. Vol. 5, N.º 2 (2020), pp. 1-9pt_PT
dc.identifier.doi10.3390/fluids5020076pt_PT
dc.identifier.issn2311-5521
dc.identifier.urihttp://hdl.handle.net/10400.21/11711
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUID/CTM/50025/2019 - FCT (CENIMAT)pt_PT
dc.relationPTDC/FIS-NAN/0117/2014 - (P.L.A.)pt_PT
dc.relationPTDC/BIA-MIC/31645/2017 - (R.G.S.)pt_PT
dc.relationUIDB/ 04378/2020 - FCT (Applied Molecular Biosciences Unit, UCIBIO)pt_PT
dc.relationPOCI-01-0145-FEDER-007728 - ERDF under the PT2020 Partnership Agreementpt_PT
dc.subjectRheologypt_PT
dc.subjectMRSApt_PT
dc.subjectS. aureuspt_PT
dc.subjectAntibioticspt_PT
dc.subjectOxacillinpt_PT
dc.subjectBactericidalpt_PT
dc.titleAntibiotic activity screened by the rheology of S. aureus culturespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage9pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleFluidspt_PT
oaire.citation.volume5pt_PT
person.familyNamePortela
person.familyNameMarques de Almeida
person.familyNameSobral
person.familyNameMarques Mendes Almeida da Rosa Leal
person.givenNameRaquel
person.givenNamePedro
person.givenNameRita
person.givenNameCatarina
person.identifierA-2814-2009
person.identifier.ciencia-id1813-219B-DD6E
person.identifier.ciencia-id0218-71B2-2DE0
person.identifier.ciencia-id7F1F-7412-A2C3
person.identifier.ciencia-idAF15-486E-BB15
person.identifier.orcid0000-0002-5559-9554
person.identifier.orcid0000-0001-7356-8455
person.identifier.orcid0000-0003-4533-7531
person.identifier.orcid0000-0001-5871-4936
person.identifier.ridB-4356-2009
person.identifier.ridH-4710-2011
person.identifier.scopus-author-id57201234562
person.identifier.scopus-author-id57190384213
person.identifier.scopus-author-id6507285825
person.identifier.scopus-author-id7005379280
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication65306061-e536-43f1-8ba0-af25087e35a1
relation.isAuthorOfPublication429b6d71-328e-42b3-8151-15493de249b8
relation.isAuthorOfPublicationef77aaab-174a-4ae6-aa70-f652bb42fc30
relation.isAuthorOfPublication4db8990d-5eb1-4515-ae7a-68873cf776a5
relation.isAuthorOfPublication.latestForDiscovery429b6d71-328e-42b3-8151-15493de249b8

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Antibiotic_PLAlmeida.pdf
Size:
902.96 KB
Format:
Adobe Portable Document Format
Download

Collections

ISEL - Física - Artigos