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Antimicrobial evaluation of the Cynara cardunculus extract in Helicobacter pylori cells using mid-infrared spectroscopy and chemometric methods

2022-09-01Journal article Restricted access
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dc.contributor.authorSampaio, Pedro
dc.contributor.authorCalado, Cecília
dc.date.accessioned2023-05-22T11:18:53Z
dc.date.available2023-05-22T11:18:53Z
dc.date.issued2022-09-01
dc.description.abstractThe treatment effectiveness of gastric diseases caused by the bacteria Helicobacter pylori is failing due to high resistance to some antibiotics. Consequently, it is urgent to develop an accurate methodology to screen new antimicrobial agents. Methods and results: A preliminary assay, using both therapeutic-based antibiotics (clarithromycin and metronidazole), was conducted to optimize experimental conditions in terms of the sensibility of the Fourier-transform mid-infrared (MIR-FTIR) spectroscopy associated with chemometric methods. Principal component analysis was applied to understand how the Cynara extract concentration acts differentially against H. pylori bacteria. The partial least squares model, characterized by R2 = 0.98, and root mean square error cross-validation, 0.011, was developed for the spectral regions (3600-2500 cm-1 and 2000-698 cm-1 ). Conclusions: MIR-FTIR spectroscopy associated with chemometric methods can be considered a suitable approach to discover and analyse the promissory antimicrobial agents based on the biomolecular changes observed according to the Cynara extract. Significance and impact of the study: MIR-FTIR spectroscopy and chemometric methods allowed to register the biomolecular changes due to the potential antimicrobial drugs at reduced concentrations comparatively to the conventional assay based on an agar-dilution method, being considered a useful approach to develop a platform to discover new bioactive molecules, allowing to reduce time and costs related to the exploratory step.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSAMPAIO, Pedro N. Sousa; CALADO, Cecília R. C. – Antimicrobial evaluation of the Cynara cardunculus extract in Helicobacter pylori cells using mid-infrared spectroscopy and chemometric methods . Journal of Applied Microbiology. ISSN 1364-5072. Vol. 133, N.º 3 (2022), pp. 1743-1756.pt_PT
dc.identifier.doi10.1111/jam.15679pt_PT
dc.identifier.eissn1365-2672
dc.identifier.issn1364-5072
dc.identifier.urihttp://hdl.handle.net/10400.21/16095
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSociety for Applied Microbiologypt_PT
dc.relationIDI&CA/IPL/2017/DrugsPlatf/ISEL - Instituto Politécnico de Lisboapt_PT
dc.relationIDI&CA/ IPL/2018/RenalProg/ISEL - Instituto Politécnico de Lisboapt_PT
dc.relationIDI&CA/IPL/2020/ NephroMD - Instituto Politécnico de Lisboapt_PT
dc.subjectHelicobacter pyloript_PT
dc.subjectCynara extractpt_PT
dc.subjectMIR-FTIR spectroscopypt_PT
dc.subjectChemometricspt_PT
dc.subjectDrug screeningpt_PT
dc.subjectHigh-throughput analysispt_PT
dc.titleAntimicrobial evaluation of the Cynara cardunculus extract in Helicobacter pylori cells using mid-infrared spectroscopy and chemometric methodspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1756pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage1743pt_PT
oaire.citation.titleJournal of Applied Microbiologypt_PT
oaire.citation.volume133pt_PT
person.familyNameSampaio
person.familyNameCalado
person.givenNamePedro
person.givenNameCecília
person.identifier1251979
person.identifier130332
person.identifier.ciencia-idAF12-6ABA-43D8
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0003-2917-4904
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id24178064100
person.identifier.scopus-author-id6603163260
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication303f3e22-ec1a-4243-9ded-98c647776e6a
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscovery303f3e22-ec1a-4243-9ded-98c647776e6a

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