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A new method for a rapid and high-throughput comparison of molecular profiles and biological activity of phytoextracts

2024-10Journal article Open access
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dc.contributor.authorCaldeira, Viviana
dc.contributor.authorFonseca, Tiago AH
dc.contributor.authorN'Dembo, Luana
dc.contributor.authorAraújo, Rúben
dc.contributor.authorVon Rekowski, Cristiana
dc.contributor.authorSampaio, Pedro
dc.contributor.authorCalado, Cecília
dc.date.accessioned2024-11-04T10:51:01Z
dc.date.available2024-11-04T10:51:01Z
dc.date.issued2024-10
dc.description.abstractTo robustly discover and explore phytocompounds, it is necessary to evaluate the interrelationships between the plant species, plant tissue, and the extraction process on the extract composition and to predict its cytotoxicity. The present work evaluated how Fourier Transform InfraRed spectroscopy can acquire the molecular profile of aqueous and ethanol-based extracts obtained from leaves, seeds, and flowers of Cynara Cardunculus, and ethanol-based extracts from Matricaria chamomilla flowers, as well the impact of these extracts on the viability of mammalian cells. The extract molecular profile enabled to predict the extraction yield, and how the plant species, plant tissue, and extraction process affected the extract's relative composition. The molecular profile obtained from the culture media of cells exposed to extracts enabled to capture its impact on cells metabolism, at a higher sensitivity than the conventional assay used to determine the cell viability. Furthermore, it was possible to detect specific impacts on the cell's metabolism according to plant species, plant tissue, and extraction process. Since spectra were acquired on small volumes of samples (25 µL), after a simple dehydration step, and based on a plate with 96 wells, the method can be applied in a rapid, simple, high-throughput, and economic mode, consequently promoting the discovery of phytocompounds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCaldeira Viviana, Fonseca Tiago AH, N'Dembo Luana, Araújo Rúben, Von Rekowski Cristiana, Sampaio Pedro, Calado Cecília RC – A new method for a rapid and high-throughput comparison of molecular profiles and biological activity of phytoextracts. Biotechnology and Bioengineering, 2024, 121(10), 28739; https://doi.org/10.1002/bit.28739pt_PT
dc.identifier.doi10.1002/bit.28739pt_PT
dc.identifier.eissn1097-0290
dc.identifier.issn0006-3592
dc.identifier.urihttp://hdl.handle.net/10400.21/17827
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/bit.28739pt_PT
dc.subjectCynara cardunculuspt_PT
dc.subjectFTIR‐spectroscopypt_PT
dc.subjectMatricaria chamomillapt_PT
dc.subjectphytocompoundspt_PT
dc.subjectpolyphenolspt_PT
dc.titleA new method for a rapid and high-throughput comparison of molecular profiles and biological activity of phytoextractspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/DSAIPA%2FDS%2F0117%2F2020/PT
oaire.citation.endPage3369pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage2981pt_PT
oaire.citation.titleBiotechnology and Bioengineeringpt_PT
oaire.citation.volume121pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameModesto Caldeira
person.familyNameHenrique Fonseca
person.familyNameAraújo
person.familyNameVon Rekowski
person.familyNameSampaio
person.familyNameCalado
person.givenNameViviana
person.givenNameTiago Alexandre
person.givenNameRúben Alexandre Dinis
person.givenNameCristiana
person.givenNamePedro
person.givenNameCecília
person.identifier1960990
person.identifier1251979
person.identifier130332
person.identifier.ciencia-idBC11-91E8-1C16
person.identifier.ciencia-id8F1D-1D48-8551
person.identifier.ciencia-id9A18-BFDC-ED95
person.identifier.ciencia-idAF12-6ABA-43D8
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-1105-056X
person.identifier.orcid0000-0003-0741-2211
person.identifier.orcid0000-0002-9369-6486
person.identifier.orcid0009-0009-6843-1935
person.identifier.orcid0000-0003-2917-4904
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id24178064100
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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