Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)

Melo, Nuno; Nimtz, Manfred; Conradt, Harald S; Fevereiro, Pedro S; Costa, Júlia

http://hdl.handle.net/10400.21/14237

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Abstract(s)

This paper reports for the first time the presence of the human Lewisa type determinant in glycoproteins secreted by plant cells. A single glycopeptide was identified in the tryptic hydrolysis of the peroxidase VMPxCl from Vaccinium myrtillus L. by HPLC/ESI-MS. The oligosaccharide structures were elucidated by ESI-MS-MS and by methylation analysis before and after removal of fucose by mild acid hydrolysis.

Keywords

Extracellular glycoprotein Peroxidase Plant complex oligosaccharide Vaccinium myrtillu

URI

http://hdl.handle.net/10400.21/14237

Citation

Melo, Nuno S, Nimtz, Manfred, Conradt, Harald S, Fevereiro, Pedro S and Costa, Júlia(1997), Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.), FEBS Letters, 415, doi: 10.1016/S0014-5793(97)01121-6