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Supercritical CO2 extracts and volatile oil of basil (Ocimum basilicum L.) comparison with conventional methods

2018-06Journal article Open access
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dc.contributor.authorCoelho, Jose
dc.contributor.authorVeiga, Jerson
dc.contributor.authorKarmali, Amin
dc.contributor.authorNicolai, Marisa
dc.contributor.authorReis, Catarina
dc.contributor.authorP. Nobre, B.
dc.contributor.authorPalavra, António
dc.date.accessioned2018-11-30T13:46:18Z
dc.date.available2018-11-30T13:46:18Z
dc.date.issued2018-06
dc.description.abstractInterest in new products from aromatic plants as medical and nutritional compounds is increasing. The aim of this work was to apply different extraction methods, including the use of supercritical carbon dioxide extraction, and to test the antioxidant activity of basil (Ocimum basilicum L.) extracts. In vitro efficacy assessments were performed using enzymatic assays. Essential oil obtained by hydrodistillation and volatile oil obtained from supercritical fluid extraction were analyzed by gas chromatography to quantify components. The total phenolic content in the extracts ranged from 35.5 +/- 2.9 to 85.3 +/- 8.6 mg of gallic acid equivalents and the total flavonoid content ranged from 35.5 +/- 2.9 to 93.3 +/- 3.9 micromole catechin equivalents per gram of dry weight of extract. All the extracts showed an antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), and the reducing power test. Extracts obtained from methanol had a higher antioxidant capacity per the DPPH test results (IC50 = 3.05 +/- 0.36 mg/mL) and the reducing power test assay 306.8 +/- 21.8 mol of trolox equivalents per gram of extract (TE/g) compared with ethanolic or supercritical fluid extracts. However, using the ABTS assay, the extract obtained by supercritical fluid extraction had a higher antioxidant capacity with an IC50 of 1.74 +/- 0.05 mg/mL. Finally, the examined extracts showed practically no acetylcholinesterase (AChE) inhibitory capacity and a slight inhibitory activity against tyrosinase.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCOELHO, José; [et al] – Supercritical CO2 extracts and volatile oil of basil (Ocimum basilicum L.) comparison with conventional methods. Separations. ISSN 2297-8739. Vol. 5, N.º 2 (2018), pp. 1-12pt_PT
dc.identifier.doi10.3390/separations5020021pt_PT
dc.identifier.issn2297-8739
dc.identifier.issn2227-9075
dc.identifier.urihttp://hdl.handle.net/10400.21/9115
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.subjectOcimum basilicumpt_PT
dc.subjectSupercritical fluid extractionpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectExtração por fluido supercríticopt_PT
dc.subjectTeor fenólico e flavonóidespt_PT
dc.titleSupercritical CO2 extracts and volatile oil of basil (Ocimum basilicum L.) comparison with conventional methodspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00100%2F2013/PT
oaire.citation.endPage12pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleSeparationspt_PT
oaire.citation.volume5pt_PT
oaire.fundingStream5876
person.familyNameCoelho
person.familyNameKarmali
person.familyNameBeco Pinto Reis
person.familyNameNobre
person.familyNamePalavra
person.givenNameJose
person.givenNameAmin
person.givenNameAna Catarina
person.givenNameBeatriz
person.givenNameAntónio
person.identifierR-000-84M
person.identifierL-2488-2014
person.identifier.ciencia-idD716-31DF-8371
person.identifier.ciencia-idA51A-0429-1EF7
person.identifier.ciencia-id1317-A7D6-3B1C
person.identifier.ciencia-idFE18-BFAF-9ED9
person.identifier.ciencia-idC21F-302E-44F7
person.identifier.orcid0000-0001-8118-0864
person.identifier.orcid0000-0003-0419-401X
person.identifier.orcid0000-0002-2490-2522
person.identifier.orcid0000-0002-1046-4031
person.identifier.orcid0000-0002-6397-9397
person.identifier.orcid0000-0003-4838-1589
person.identifier.ridG-2626-2011
person.identifier.ridE-4787-2011
person.identifier.ridJ-3977-2013
person.identifier.ridH-9235-2012
person.identifier.scopus-author-id7101841005
person.identifier.scopus-author-id6603778216
person.identifier.scopus-author-id36864455300
person.identifier.scopus-author-id6602872178
person.identifier.scopus-author-id6701681759
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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