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Monitoring the ex-vivo expansion of human mesenchymal stem/stromal cells in xeno-free microcarrier-based reactor systems by MIR spectroscopy

dc.contributor.authorRosa, Filipa
dc.contributor.authorSales, Kevin C.
dc.contributor.authorCarmelo, Joana G.
dc.contributor.authorFernandes-Platzgummer, Ana
dc.contributor.authorSilva, Cláudia L. da
dc.contributor.authorLopes, Marta B.
dc.contributor.authorCalado, Cecília
dc.date.accessioned2016-04-20T16:12:00Z
dc.date.available2016-04-20T16:12:00Z
dc.date.issued2016-02-12
dc.description.abstractHuman mesenchymal stem/stromal cells (MSCs) have received considerable attention in the field of cell-based therapies due to their high differentiation potential and ability to modulate immune responses. However, since these cells can only be isolated in very low quantities, successful realization of these therapies requires MSCs ex-vivo expansion to achieve relevant cell doses. The metabolic activity is one of the parameters often monitored during MSCs cultivation by using expensive multi-analytical methods, some of them time-consuming. The present work evaluates the use of mid-infrared (MIR) spectroscopy, through rapid and economic high-throughput analyses associated to multivariate data analysis, to monitor three different MSCs cultivation runs conducted in spinner flasks, under xeno-free culture conditions, which differ in the type of microcarriers used and the culture feeding strategy applied. After evaluating diverse spectral preprocessing techniques, the optimized partial least square (PLS) regression models based on the MIR spectra to estimate the glucose, lactate and ammonia concentrations yielded high coefficients of determination (R2 ≥ 0.98, ≥0.98, and ≥0.94, respectively) and low prediction errors (RMSECV ≤ 4.7%, ≤4.4% and ≤5.7%, respectively). Besides PLS models valid for specific expansion protocols, a robust model simultaneously valid for the three processes was also built for predicting glucose, lactate and ammonia, yielding a R2 of 0.95, 0.97 and 0.86, and a RMSECV of 0.33, 0.57, and 0.09 mM, respectively. Therefore, MIR spectroscopy combined with multivariate data analysis represents a promising tool for both optimization and control of MSCs expansion processes.pt_PT
dc.identifier.citationROSA, Filipa; [et al.] - Monitoring the ex-vivo expansion of human mesenchymal stem/stromal cells in xeno-free microcarrier-based reactor systems by MIR spectroscopy. Biotechnology Progress. ISSN. 1520-6033. Vol. 32, N.º 2 (2016), pp. 447-455pt_PT
dc.identifier.doi10.1002/btpr.2215pt_PT
dc.identifier.issn1520-6033
dc.identifier.urihttp://hdl.handle.net/10400.21/6061
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWILEYpt_PT
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/btpr.2215/abstractpt_PT
dc.subjectMesenchymal stem/stromal cellspt_PT
dc.subjectMIR spectroscopypt_PT
dc.subjectHigh-throughput analysispt_PT
dc.titleMonitoring the ex-vivo expansion of human mesenchymal stem/stromal cells in xeno-free microcarrier-based reactor systems by MIR spectroscopypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F73758%2F2010/PT
oaire.citation.endPage455
oaire.citation.issue2pt_PT
oaire.citation.startPage447
oaire.citation.volume32pt_PT
oaire.fundingStreamSFRH
person.familyNameCalado
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc
relation.isProjectOfPublication2e866b8b-53df-4729-b132-92adf509db2c
relation.isProjectOfPublication.latestForDiscovery2e866b8b-53df-4729-b132-92adf509db2c

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