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Chitosan nanoparticles for enhanced immune response and delivery of multi-epitope helicobacter pylori vaccines in a BALB/c mouse model

2025-01-18Research article Open access
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authorProfile.emailbiblioteca@isel.pt
datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
dc.contributor.authorAmaral, Rita
dc.contributor.authorConcha, Tomás
dc.contributor.authorVítor, Jorge
dc.contributor.authorAlmeida, António J.
dc.contributor.authorCalado, Cecília
dc.contributor.authorDiogo Gonçalves, Lídia Maria
dc.date.accessioned2025-04-02T09:46:36Z
dc.date.available2025-04-02T09:46:36Z
dc.date.issued2025-01-18
dc.description.abstractHelicobacter pylori is the leading cause of chronic gastritis, peptic ulcer, gastric adenocarcinoma, and mucosal-associated lymphoma. Due to the emerging problems with antibiotic treatment against H. pylori in clinical practice, H. pylori vaccination has gained more interest. Oral immunization is considered a promising approach for preventing initial colonization of this bacterium in the gastrointestinal tract, establishing a first line of defense at gastric mucosal surfaces. Chitosan nanoparticles can be exploited effectively for oral vaccine delivery due to their stability, simplicity of target accessibility, and beneficial mucoadhesive and immunogenic properties. Methods: In this study, new multi-epitope pDNA- and recombinant protein-based vaccines incorporating multiple H. pylori antigens were produced and encapsulated in chitosan nanoparticles for oral and intramuscular administration. The induced immune response was assessed through the levels of antigen-specific IgGs, secreted mucosal SIgA, and cytokines (IL-2, IL-10, and IFN-γ) in immunized BALB/C mice. Results: Intramuscular administration of both pDNA and recombinant protein-based vaccines efficiently stimulated the production of specific IgG2a and IgG1, which was supported by cytokines levels. Oral immunizations with either pDNA or recombinant protein vaccines revealed high SIgA levels, suggesting effective gastric mucosal immunization, contrasting with intramuscular immunizations, which did not induce SIgA. Conclusions: These findings indicate that both pDNA and recombinant protein vaccines encapsulated into chitosan nanoparticles are promising candidates for eradicating H. pylori and mitigating associated gastric diseases in humans.por
dc.identifier.citationAmaral, R., Concha, T., Vítor, J., Almeida, A. J., Calado, C., & Gonçalves, L. M. (2025). Chitosan nanoparticles for enhanced immune response and delivery of multi-epitope helicobacter pylori vaccines in a BALB/c mouse model. Pharmaceutics, 17(1), 132. https://doi.org/10.3390/pharmaceutics17010132
dc.identifier.doihttps://doi.org/10.3390/pharmaceutics17010132
dc.identifier.eissn1999-4923
dc.identifier.urihttp://hdl.handle.net/10400.21/21732
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationPTDC/BIO/69242/2006 - Fundação para a Ciência e a Tecnologia (FCT)
dc.relationPest-UIDB/04138/2020 - Fundação para a Ciência e a Tecnologia (FCT)
dc.relationUIDP/04138/2020 - Fundação para a Ciência e a Tecnologia (FCT)
dc.relationCEECIND/03143/2017 - Fundação para a Ciência e a Tecnologia (FCT)
dc.relation.hasversionhttps://www.mdpi.com/1999-4923/17/1/132
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectHelicobacter pylori
dc.subjectVaccine
dc.subjectDNA vaccine
dc.subjectImmunization
dc.subjectRecombinant antigens
dc.titleChitosan nanoparticles for enhanced immune response and delivery of multi-epitope helicobacter pylori vaccines in a BALB/c mouse modeleng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.endPage20
oaire.citation.issue1
oaire.citation.startPage1
oaire.citation.titlePharmaceutics
oaire.citation.volume17
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCalado
person.familyNameDiogo Gonçalves
person.givenNameCecília
person.givenNameLídia Maria
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-id7211-22BA-86AD
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-6799-2740
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication3f8540f5-bc6b-4e10-94c5-7d0325b7fa3f
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc

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