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Purification and partial characterization of peroxidases from three food waste by-products: broad bean pods, pea pods, and artichoke stems

2019-10Journal article Restricted access
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dc.contributor.authorMEJRI, Faiza
dc.contributor.authorKarmali, Amin
dc.contributor.authorJaoued, Najeh
dc.contributor.authorCasabianca, Herve
dc.contributor.authorHosni, Karim
dc.date.accessioned2019-10-21T09:11:44Z
dc.date.available2019-10-21T09:11:44Z
dc.date.issued2019-10
dc.description.abstractIn this study, peroxidases (PODs) from three waste by-products: broad bean pods (BBP), pea pods (PP), and artichoke stems (ARS) were purified and their optimal conditions were determined for the first time. The purification process resulted in 4.32, 7.21, and 8.9% of POD recoveries for PP, ARS, and BBP, respectively. They were purified 2.12-, 32.97-, and 10-fold with specific activities of 27.26, 266.43, and 27 U/mg of protein, respectively. Analysis of their optimal conditions showed that POD purified from BBP and PP exhibited the highest activity at 60 degrees C temperature and pH 6 and 8 with strong affinity with catechol substrate (Km of 0.356 and 0.189 mM; Vmax of 0.08 and 0.041 mu M/min for BBP and PP, respectively). The highest activity of ARS POD was obtained under the following conditions: temperature at 50 degrees C, pH from 6 to 8, and guaiacol as substrate (Km 0.375 mM; Vmax 0.012 mu M/min). Apart from giving the opportunity for recycling the food industry wastes, the studied waste by-products could represent an alternative source of PODs that could find several applications in the biotechnological, chemical, and food industries.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMEJRI, Faiza; [et al] – Purification and partial characterization of peroxidases from three food waste by-products: broad bean pods, pea pods, and artichoke stems. Applied Biochemistry and Biotechnology. ISSN 0273-2289. Vol. 189, N.º 2 (2019), pp. 576-588pt_PT
dc.identifier.doi10.1007/s12010-019-03028-8pt_PT
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.urihttp://hdl.handle.net/10400.21/10590
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/content/pdf/10.1007%2Fs12010-019-03028-8.pdfpt_PT
dc.subjectBroad bean podspt_PT
dc.subjectPea podspt_PT
dc.subjectArtichoke stemspt_PT
dc.subjectPeroxidasespt_PT
dc.subjectWaste by-productspt_PT
dc.subjectValorizationpt_PT
dc.titlePurification and partial characterization of peroxidases from three food waste by-products: broad bean pods, pea pods, and artichoke stemspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage588pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage576pt_PT
oaire.citation.titleApplied Biochemistry and Biotechnologypt_PT
oaire.citation.volume189pt_PT
person.familyNameMEJRI
person.familyNameKarmali
person.familyNamejaoued
person.familyNamehosni
person.givenNameFaiza
person.givenNameAmin
person.givenNamenajeh
person.givenNamekarim
person.identifier.orcid0000-0003-2846-2461
person.identifier.orcid0000-0003-0419-401X
person.identifier.orcid0000-0003-4759-4662
person.identifier.orcid0000-0003-0978-8378
person.identifier.ridE-4787-2011
person.identifier.scopus-author-id56682005900
person.identifier.scopus-author-id6603778216
person.identifier.scopus-author-id15834455400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication599a4113-42a8-4bd3-9fe7-1a4dedca703e
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublicationbcb8196d-1e9d-43a1-9697-28b25cd86d71
relation.isAuthorOfPublication6d5da6b3-484c-4e47-9220-ec7122fa5d54
relation.isAuthorOfPublication.latestForDiscovery599a4113-42a8-4bd3-9fe7-1a4dedca703e

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