Repository logo
 
Publication

Optimisation of culture conditions and characterisation of cutinase produced by recombinant Saccharomyces cerevisiae

2002-07-01Journal article Restricted access
Simple item page
dc.contributor.authorCalado, Cecília
dc.contributor.authorTaipa, M. Ângela
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorFonseca, Luís P. P.
dc.date.accessioned2020-10-02T10:24:05Z
dc.date.available2020-10-02T10:24:05Z
dc.date.issued2002-07-01
dc.description.abstractThe optimum culture dissolved oxygen concentration and culture pH for the production of cutinase from Fusarium solani pisi by the recombinant Saccharomyces cerevisiae SU50 strain was investigated. Dissolved oxygen concentrations in the fermentation culture of 5, 30 and 60% of air saturation were evaluated. A high cutinase production, specific cutinase activity and the highest cutinase yield on biomass and the highest specific cutinase production rate were obtained with a 5% of air saturation, which could have impact on process economics. Furthermore, at a low dissolved oxygen concentration, the specific growth rate, specific cutinase production rate, cutinase yield on biomass, cutinase activity and specific cutinase activity were increased when the pH control was changed from 5.25 to 6.25. The cutinase, accumulated in the yeast culture presents two glycosilated isoforms with molecular weights of 22.8 and 24.9 kDa measured by SDS-PAGE. Furthermore, cutinase in clarified culture samples presents a linear relationship between estereolytic and lypolitic activity and a high stability at room temperature.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCALADO, Cecília R. C.; [et al] – Optimisation of culture conditions and characterisation of cutinase produced by recombinant Saccharomyces cerevisiae. Enzyme and Microbial Technology. ISSN 0141-0229. Vol. 31, N.ºs 1-2 (2002), pp. 161-170pt_PT
dc.identifier.doi10.1016/S0141-0229(02)00084-4pt_PT
dc.identifier.issn0141-0229
dc.identifier.urihttp://hdl.handle.net/10400.21/12272
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPRAXIS XXI, Ministério da Ciência e Tecnologia, Portugal (BD/18276/98)pt_PT
dc.relationEU Project: Integrated Bioprocess Design for Large Scale Production and Isolation of Recombinant Proteins, BIO4-CT96-0435pt_PT
dc.subjectCulture conditionspt_PT
dc.subjectCutinasept_PT
dc.subjectFusarium solani pisipt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.titleOptimisation of culture conditions and characterisation of cutinase produced by recombinant Saccharomyces cerevisiaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage170pt_PT
oaire.citation.issue1-2pt_PT
oaire.citation.startPage161pt_PT
oaire.citation.titleEnzyme and Microbial Technologypt_PT
oaire.citation.volume31pt_PT
person.familyNameCalado
person.familyNameCabral
person.familyNameP. Fonseca
person.givenNameCecília
person.givenNameJoaquim M.S.
person.givenNameLuis
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-idAB13-9ED3-C821
person.identifier.ciencia-id951D-DF38-3397
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-2405-5845
person.identifier.orcid0000-0001-8429-6977
person.identifier.ridE-2102-2014
person.identifier.ridG-2052-2010
person.identifier.ridA-4228-2013
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id7201350203
person.identifier.scopus-author-id55916288400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication596986f6-6f41-4107-a097-bc205ecdb95a
relation.isAuthorOfPublication5c533551-5113-4c0a-93f1-9c50cbd796bc
relation.isAuthorOfPublication.latestForDiscovery596986f6-6f41-4107-a097-bc205ecdb95a

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Optimisation_CRCCalado.pdf
Size:
350 KB
Format:
Adobe Portable Document Format
Download

Collections

ISEL - Eng. Quim. Biol. - Artigos