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Bioconversion of D-glucose into D-glucosone by immobilized glucose 2-oxidase from Coriolus versicolorat moderate pressures

2011-01Journal article Open access
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dc.contributor.authorKarmali, Amin
dc.contributor.authorCoelho, Jose
dc.date.accessioned2013-01-30T19:13:33Z
dc.date.available2013-01-30T19:13:33Z
dc.date.issued2011-01
dc.description.abstractThe immobilized glucose 2-oxidase (pyranose oxidase, pyranose:oxygen-2-oxidoreductase, EC 1.1.3.10) from Coriolus versicolor was used to convert D-glucose into D-glucosone at moderate pressures, up to 150 bar, with compressed air in a modified commercial batch reactor. Several parameters affecting biocatalysis at moderate pressures were investigated as follows: pressure, different forms of immobilized biocatalysts, glucose concentration, pH, temperature and the presence of catalase. Glucose 2-oxidase (GOX2) was purified by immobilized metal affinity chromatography on epoxy-activated Sepharose 6B-IDA-Cu(II) column at pH 6.0. Purified enzyme and catalase were immobilized into a polyethersulfone (PES) membrane in the presence of glutaraldehyde and gelatin. Enhancement of the bioconversion of D-glucose was done by the pressure since an increase in the pressure with compressed air increases the conversion rates. The optimum temperature and pH for bioconversion of D-glucose were found to be 62 degrees C and pH 6.0, respectively and the activation energy (E(a)) was 28.01 kJ mol(-1). The apparent kinetic constants (V(max)' K(m)', K(cat)' and K(cat)/K(m)') for this bioconversion were 2.27 U mg(-1) protein, 11.15 mM, 8.33 s(-1) and 747.38 s(-1) M(-1), respectively. The immobilized biomass of C. versicolor as well as crude extract containing GOX2 activity were also useful for bioconversion of D-glucose at 65 bar with a yield of 69.9 +/- 3.8% and 91.3 +/- 1.2%, respectively. The immobilized enzyme was apparently stable for several months without any significant loss of enzyme activity. On the other hand, this immobilized enzyme was also stable at moderate pressures, since such pressures did not affect significantly the enzyme activity. (C) 2010 Elsevier Ltd. All rights reserved.por
dc.identifier.citationKARMALI, Amin; COELHO, José - Bioconversion of D-glucose into D-glucosone by immobilized glucose 2-oxidase from Coriolus versicolorat moderate pressures. Process Biochemistry. ISSN 1359-5113. Vol. 46, n.º 1 (2011) p. 168-173.por
dc.identifier.issn1359-5113
dc.identifier.urihttp://hdl.handle.net/10400.21/2110
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier SCI LTDpor
dc.subjectBiocatalysis at Moderate Pressurespor
dc.subjectImmobilized Glucose 2-Oxidase from Coriolus versicolorpor
dc.subjectImmobilization in polyethersulfone membranespor
dc.subjectCatalasepor
dc.subjectBioconversion of D-glucose into D-Glucosonepor
dc.subjectApparent kinetic constantspor
dc.subjectPyranose 2-oxidasepor
dc.subjectChromatographic behaviorpor
dc.subjectEnzymatic oxidationpor
dc.subjectFungal strainspor
dc.subjectOxidasepor
dc.subjectPurificationpor
dc.subjectProductspor
dc.subjectEnzymespor
dc.subjectDesignpor
dc.titleBioconversion of D-glucose into D-glucosone by immobilized glucose 2-oxidase from Coriolus versicolorat moderate pressurespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceOxonpor
oaire.citation.endPage173por
oaire.citation.issue1por
oaire.citation.startPage168por
oaire.citation.titleProcess Biochemistrypor
oaire.citation.volume46por
person.familyNameKarmali
person.familyNameCoelho
person.givenNameAmin
person.givenNameJose
person.identifierR-000-84M
person.identifier.ciencia-idD716-31DF-8371
person.identifier.orcid0000-0003-0419-401X
person.identifier.orcid0000-0001-8118-0864
person.identifier.ridE-4787-2011
person.identifier.ridG-2626-2011
person.identifier.scopus-author-id6603778216
person.identifier.scopus-author-id7101841005
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublicationec13b922-f442-44fa-bb07-7c2e0bebbe6d
relation.isAuthorOfPublication.latestForDiscovery23975b4b-3234-4139-9675-89ea6f9a2332

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