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Wasteful azo dyes as a source of biologically active building blocks

2021-06-15Journal article Open access
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dc.contributor.authorFernandes, Ana
dc.contributor.authorPinto, Bruna
dc.contributor.authorBonardo, Lorenzo
dc.contributor.authorRoyo, Beatriz
dc.contributor.authorRobalo, M. Paula
dc.contributor.authorMartins, Lígia O.
dc.date.accessioned2021-10-07T14:59:57Z
dc.date.available2021-10-07T14:59:57Z
dc.date.issued2021-06-15
dc.description.abstractIn this work, an environment-friendly enzymatic strategy was developed for the valorisation of dye-containing wastewaters. We set up biocatalytic processes for the conversion of azo dyes representative of the main classes used in the textile industry into valuable aromatic compounds: aromatic amines, phenoxazinones, phenazines, and naphthoquinones. First, purified preparations of PpAzoR azoreductase efficiently reduced mordant, acid, reactive, and direct azo dyes into aromatic amines, and CotA-laccase oxidised these compounds into phenazines, phenoxazinones, and naphthoquinones. Second, whole cells containing the overproduced enzymes were utilised in the two-step enzymatic conversion of the model mordant black 9 dye into sodium 2-amino-3-oxo-3H-phenoxazine-8-sulphonate, allowing to overcome the drawbacks associated with the use of expensive purified enzymes, co-factors, or exquisite reaction conditions. Third, cells immobilised in sodium alginate allowed recycling the biocatalysts and achieving very good to excellent final phenoxazine product yields (up to 80%) in water and with less impurities in the final reaction mixtures. Finally, one-pot systems using recycled immobilised cells co-producing both enzymes resulted in the highest phenoxazinone yields (90%) through the sequential use of static and stirring conditions, controlling the oxygenation of reaction mixtures and the successive activity of azoreductase (anaerobic) and laccase (aerobic).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFERNANDES, Ana; [et al] – Wasteful azo dyes as a source of biologically active building blocks. Frontiers in Bioengineering and Biotechnology. ISSN 2296-4185. Vol. 9 (2021), pp. 1-9pt_PT
dc.identifier.doi10.3389/fbioe.2021.672436pt_PT
dc.identifier.issn2296-4185
dc.identifier.urihttp://hdl.handle.net/10400.21/13841
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFRONTIERS MEDIA SApt_PT
dc.relationUID/Multi/045 51/2013 - FEDER funds through COMPETE2020-Programa Operacional Competitividade e Internacionalizacao (POCI)pt_PT
dc.relationLISBOA-01-0145-FEDER-007660 - FEDER funds through COMPETE2020-Programa Operacional Competitividade e Internacionalizacao (POCI)pt_PT
dc.subjectLaccasespt_PT
dc.subjectAromatic aminespt_PT
dc.subjectWhole-cell catalysispt_PT
dc.subjectPhenazinespt_PT
dc.subjectPhenoxazinonespt_PT
dc.subjectAzoreductasept_PT
dc.titleWasteful azo dyes as a source of biologically active building blockspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-EBB%2F0122%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBII-BBF%2F29564%2F2017/PT
oaire.citation.endPage9pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleFrontiers in Bioengineering and Biotechnologypt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
person.familyNameRoyo Cantabrana
person.familyNameRobalo
person.familyNameMartins
person.givenNameBeatriz
person.givenNameMaria Paula
person.givenNameLígia O.
person.identifierB-6992-2008
person.identifier319225
person.identifier.ciencia-id7012-5853-4390
person.identifier.ciencia-idE11C-A704-9C18
person.identifier.ciencia-id731B-B843-C1DF
person.identifier.orcid0000-0002-7909-9992
person.identifier.orcid0000-0002-8200-6910
person.identifier.orcid0000-0003-0082-9591
person.identifier.ridB-2002-2012
person.identifier.ridC-2513-2009
person.identifier.scopus-author-id6602728964
person.identifier.scopus-author-id9269662100
person.identifier.scopus-author-id7102109314
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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