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Production, purification and characterisation of polysaccharides from pleurotus ostreatus with antitumor activity

2012-07Journal article Restricted access
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dc.contributor.authorSilva, Sara
dc.contributor.authorMartins, Sónia
dc.contributor.authorKarmali, Amin
dc.contributor.authorRosa, Eduardo
dc.date.accessioned2015-09-08T15:10:12Z
dc.date.available2015-09-08T15:10:12Z
dc.date.issued2012-07
dc.description.abstractBackground: Mushroom polysaccharides play an important role in functional foods because they exhibit biological modulator properties such as antitumour, antiviral and antibacterial activities. The present study involved the production, purification and characterisation of intracellular and extracellular free and protein-bound polysaccharides from Pleurotus ostreatus and the investigation of their growth-inhibitory effect on human carcinoma cell lines. Results: Several fermentation parameters were obtained: batch polysaccharide productivities of 0.013 +/- 8.12 x 10-5 and 0.037 +/- 0.0005 g L-1 day-1 for intracellular and extracellular polysaccharides respectively, a maximum biomass concentration of 9.35 +/- 0.18 g L-1, Pmax = 0.935 +/- 0.018 g L-1 day-1, µmax = 0.218 +/- 0.02 day-1, YEP/X = 0.040 +/- 0.0015 g g-1 and YIP/X = 0.014 +/- 0.0003 g g-1. Some polysaccharides exhibited superoxide dismutase (SOD)-like activity of 50-200 units. Fourier transform infrared analysis of the polysaccharides revealed absorption bands characteristic of such biological macromolecules. Cytotoxicity assays showed that both intracellular and extracellular polysaccharides exhibited antitumour activity towards several tested human carcinoma cell lines in a dose-dependent manner. Conclusion - The polysaccharides of P. ostreatus exhibited high SOD-like activity, which strongly supports their biological effect on tumour cell lines. The extracellular polysaccharides presented the highest antitumour activity towards the RL95 carcinoma cell line and should be further investigated as an antitumour agent.por
dc.identifier.citationSILVA, S.; MARTINS, S.; KARMALI, A.; ROSA, E. – Production, purification and characterisation of polysaccharides from pleurotus ostreatus with antitumor activity. Journal of the Science of Food and Agriculture. ISSN: 0022-5142. Vol. 92, nr. 9 (2012), pp. 1826-1832por
dc.identifier.doi10.1002/jsfa.5560
dc.identifier.issn0022-5142
dc.identifier.urihttp://hdl.handle.net/10400.21/5115
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWiley-Blackwellpor
dc.relationFundação para a Ciência e a Tecnologia (Unit 702), Lisbon (Portugal) - PTDC/AGR-AAM/74526/2006
dc.relationFCT - PTDC/AGR-AAM/74526/2006
dc.subjectPolysaccharidespor
dc.subjectPleurotus ostreatuspor
dc.subjectSubmerged Fermentationpor
dc.subjectHPLCpor
dc.subjectFTIRpor
dc.subjectGel Filtration Chromatographypor
dc.subjectSOD-Like Activitypor
dc.subjectIn vitro Antitumour Activitypor
dc.subjectFruiting Body
dc.subjectMushrooms
dc.subjectCulture
dc.titleProduction, purification and characterisation of polysaccharides from pleurotus ostreatus with antitumor activitypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceHoboken
oaire.citation.endPage1832por
oaire.citation.issue9por
oaire.citation.startPage1826por
oaire.citation.titleJournal of the Science of Food and Agriculturepor
oaire.citation.volume92por
person.familyNameKarmali
person.givenNameAmin
person.identifier.orcid0000-0003-0419-401X
person.identifier.ridE-4787-2011
person.identifier.scopus-author-id6603778216
rcaap.rightsclosedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublication.latestForDiscovery23975b4b-3234-4139-9675-89ea6f9a2332

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