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Molecular-level changes induced by hydroxycinnamic acid derivatives in HepG2 cell line: comparison with pravastatin

2021-10-15Journal article Restricted access
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dc.contributor.authorRESSAISSI, Asma
dc.contributor.authorPacheco, Rita
dc.contributor.authorSerralheiro, Maria Luisa
dc.date.accessioned2021-10-14T14:12:06Z
dc.date.available2021-10-14T14:12:06Z
dc.date.issued2021-10-15
dc.description.abstractHydroxycinnamic acid derivatives are an important class of polyphenols found in fruits, vegetables, and medicinal plants and widely consumed in human diet. In the present work, alterations of HepG2 cells biochemical profile under the effect of four hydroxycinnamic acid derivatives (caffeic acid, m-coumaric acid, chlorogenic acid and rosmarinic acid) relatively to the effect of pravastatin, a drug often prescribed to inhibit HMG-CoA reductase enzyme, the regulator enzyme in the cholesterol biosynthesis pathway, were reported. The application of FTIR spectroscopy in combination with multivariate analysis by PCA showed a similarity between pravastatin and the four hydroxycinnamic acid derivatives in metabolite profile modification expressed by various changes in proteins region, the phosphate region which mainly corresponds to nucleic acids as well as in lipids regions. FTIR structural analysis in the amide I region, using resolution enhancement methods, such as second derivative and amide I deconvolution method, revealed significant decrease in alpha-helix/random coil and intermolecular beta-sheet decreased while intramolecular beta-sheet in treated cells showed an increase. It was also noticed that the intracellular cholesterol as well as esterified ingredients such as cholesterol esters in the cell membrane decreased. Moreover, principal component analysis (PCA) of the spectral data showed that the compounds and pravastatin were well separated from untreated cells showing a different mode of action on HepG2 treated cells for each compound.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRESSAISSI, Asma; PACHECO, Rita; SERRALHEIRO, Maria Luísa M. – Molecular-level changes induced by hydroxycinnamic acid derivatives in HepG2 cell line: comparison with pravastatin. Life Sciences. ISSN 0024-3205. Vol. 283 (2021), pp. 1-11pt_PT
dc.identifier.doi10.1016/j.lfs.2021.119846pt_PT
dc.identifier.eissn1879-0631
dc.identifier.issn0024-3205
dc.identifier.urihttp://hdl.handle.net/10400.21/13892
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationBiosystems & Integrative Sciences Institute
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S002432052100833Xpt_PT
dc.subjectHydroxycinnamic acid derivativespt_PT
dc.subjectPravastatinpt_PT
dc.subjectHepG2pt_PT
dc.subjectFTIR spectroscopypt_PT
dc.subjectPCApt_PT
dc.titleMolecular-level changes induced by hydroxycinnamic acid derivatives in HepG2 cell line: comparison with pravastatinpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBiosystems & Integrative Sciences Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04046%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BQM%2F28355%2F2017/PT
oaire.citation.endPage11pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleLife Sciencespt_PT
oaire.citation.volume283pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNameRESSAISSI
person.familyNamePacheco
person.familyNameSerralheiro
person.givenNameAsma
person.givenNameRita
person.givenNameMaria Luisa
person.identifier1022927
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.orcid0000-0002-0073-6785
person.identifier.orcid0000-0001-5192-3006
person.identifier.orcid0000-0001-7541-9613
person.identifier.ridC-3062-2012
person.identifier.ridE-3407-2012
person.identifier.scopus-author-id8853708300
person.identifier.scopus-author-id6602688573
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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