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Detection of FRET signals with a wavelength sensitive device based on a-SiC:H

2013-06-15Journal article Open access
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dc.contributor.authorLouro, Paula
dc.contributor.authorVieira, Manuela
dc.contributor.authorCosta, João
dc.contributor.authorVaz da Silva, V
dc.contributor.authorPatriarca, João
dc.contributor.authorKarmali, Amin
dc.date.accessioned2013-11-02T18:48:22Z
dc.date.available2013-11-02T18:48:22Z
dc.date.issued2013-06-15
dc.description.abstractGlucose sensing is an issue with great interest in medical and biological applications. One possible approach to glucose detection takes advantage of measuring changes in fluorescence resonance energy transfer (FRET) between a fluorescent donor and an acceptor within a protein which undergoes glucose-induced changes in conformation. This demands the detection of fluorescent signals in the visible spectrum. In this paper we analyzed the emission spectrum obtained from fluorescent labels attached to a protein which changes its conformation in the presence of glucose using a commercial spectrofluorometer. Different glucose nanosensors were used to measure the output spectra with fluorescent signals located at the cyan and yellow bands of the spectrum. A new device is presented based on multilayered a-SiC:H heterostructures to detect identical transient visible signals. The transducer consists of a p-i'(a-SiC:H)-n/p-i(a-Si:H)-n heterostructure optimized for the detection of the fluorescence resonance energy transfer between fluorophores with excitation in the violet (400 nm) and emissions in the cyan (470 nm) and yellow (588 nm) range of the spectrum. Results show that the device photocurrent signal measured under reverse bias and using appropriate steady state optical bias, allows the separate detection of the cyan and yellow fluorescence signals. (C) 2013 Elsevier B.V. All rights reserved.por
dc.identifier.citationLOURO, P.; VIEIRA, M.; COSTA, J.; SILVA, V.; PATRIARCA, J.; KARMALI, A. - Detection of FRET signals with a wavelength sensitive device based on a-SiC:H. Applied Surface Science. ISSN 0169-4332. Vol. 275 (2013), p. 49-53.por
dc.identifier.issn0169-4332
dc.identifier.other10.1016/j.apsusc.2013.01.126
dc.identifier.urihttp://hdl.handle.net/10400.21/2845
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier Science BVpor
dc.subjectSiC hetrostructurespor
dc.subjectOptical sensorspor
dc.subjectFluorescence proteinspor
dc.subjectGlucose nanosensorspor
dc.titleDetection of FRET signals with a wavelength sensitive device based on a-SiC:Hpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceAmsterdampor
oaire.citation.endPage53por
oaire.citation.startPage49por
oaire.citation.titleApplied Surface Sciencepor
oaire.citation.volume275por
person.familyNameLouro
person.familyNameVieira
person.familyNameVaz da Silva
person.familyNameKarmali
person.givenNamePaula
person.givenNameManuela
person.givenNameVitor
person.givenNameAmin
person.identifier499161
person.identifier10792
person.identifier.ciencia-idE511-8C08-E606
person.identifier.ciencia-id9516-E25E-BB8E
person.identifier.ciencia-idAB18-740E-B580
person.identifier.orcid0000-0002-4167-2052
person.identifier.orcid0000-0002-1150-9895
person.identifier.orcid0000-0003-4265-6553
person.identifier.orcid0000-0003-0419-401X
person.identifier.ridU-8346-2017
person.identifier.ridV-7860-2017
person.identifier.ridE-4787-2011
person.identifier.scopus-author-id8845716400
person.identifier.scopus-author-id7202140173
person.identifier.scopus-author-id6603778216
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication730dc3dd-73eb-428d-b3a6-430085e33585
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relation.isAuthorOfPublicationbf73931d-fd0b-44bc-82a9-fdfd2157406f
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublication.latestForDiscovery730dc3dd-73eb-428d-b3a6-430085e33585

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