Repository logo
 
Publication

Impact of the human mesenchymal stem cells donor on conditional medium composition

2019-04-18Conference object Restricted access
Simple item page
dc.contributor.authorPereira, Maria João Canha
dc.contributor.authorRamalhete, Luís
dc.contributor.authorAleixo, Sandra
dc.contributor.authorda Silva, Cláudia L.
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorCalado, Cecília
dc.contributor.authorFernandes, Ana
dc.date.accessioned2019-05-15T10:02:32Z
dc.date.available2019-05-15T10:02:32Z
dc.date.issued2019-04-18
dc.description.abstractExosomes produced by Mesenchymal Stem Cells (MSCs) can represent a very appealing strategy for cell-free therapies. However, to achieve this reality it is necessary to further understand the process associated to the MSC culture when conditioned to produce exosomes. In the present work, it was evaluated how different MSC obtained from different donors may affect the conditioned media composition and how this can be influenced by the conditioned media type (DMEM versus Xeno-Free medium, XF). The molecular fingerprint of the conditioned media composition was obtained by mid-infrared (MIR) spectroscopy, as this technique reflects fundamental vibrations of a high diversity of functional chemical groups present in biological samples. It was observed by principal component analysis of the second derivative spectra of conditioned media that the media chemical composition depends more from the MSCs donor than the conditioning days. Diverse spectral regions, characteristic of defined chemical groups, enabled to discriminate the chemical composition of the media according to the MSC donor. All of this was observed in both types of media (DMEM and XF). This work is a step forward to understand how different MSC donors and conditioned media may affect the exosomes characteristics.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPEREIRA, Maria João Canha; [et al] – Impact of the human mesenchymal stem cells donor on conditional medium composition. In 2019 IEEE 6th Portuguese Meeting on Bioengineering (ENBENG). Lisbon, Portugal: IEEE, 2019. ISBN 978-1-5386-8506-8. Pp. 1-4pt_PT
dc.identifier.doi10.1109/ENBENG.2019.8692511pt_PT
dc.identifier.isbn978-1-5386-8506-8
dc.identifier.isbn978-1-5386-8507-5
dc.identifier.urihttp://hdl.handle.net/10400.21/10018
dc.language.isoengpt_PT
dc.publisherInstitute of Electrical and Electronics Engineerspt_PT
dc.relationPTDC/QEQ-EPR/6623/2014 - FCTpt_PT
dc.relationPTDC/EQU-EQU/31651/2017 - FCTpt_PT
dc.relation.publisherversionhttps://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=8692511pt_PT
dc.subjectMesenchymal stem cellspt_PT
dc.subjectConditioned mediapt_PT
dc.subjectMolecular fingerprintpt_PT
dc.titleImpact of the human mesenchymal stem cells donor on conditional medium compositionpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04565%2F2013/PT
oaire.citation.conferencePlace22-23 Feb. 2019 - Lisbon, Portugalpt_PT
oaire.citation.endPage4pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.title2019 IEEE 6th Portuguese Meeting on Bioengineering (ENBENG)pt_PT
oaire.fundingStream5876
person.familyNameRamalhete
person.familyNameAleixo
person.familyNameCabral
person.familyNameCalado
person.familyNameFernandes-Platzgummer
person.givenNameLuís
person.givenNameSandra
person.givenNameJoaquim M.S.
person.givenNameCecília
person.givenNameAna
person.identifier2296066
person.identifier130332
person.identifier657490
person.identifier.ciencia-idDF19-022D-AA10
person.identifier.ciencia-idAB13-9ED3-C821
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-idEA11-6777-F573
person.identifier.orcid0000-0002-8911-3380
person.identifier.orcid0000-0003-1740-8371
person.identifier.orcid0000-0002-2405-5845
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-5367-6528
person.identifier.ridG-2052-2010
person.identifier.ridE-2102-2014
person.identifier.ridF-1685-2011
person.identifier.scopus-author-id57208672678
person.identifier.scopus-author-id24829443600
person.identifier.scopus-author-id7201350203
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id35749894500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication7846e088-851e-47b6-a7bd-d02c9d8f047d
relation.isAuthorOfPublicationc7cbebb9-e51f-4f99-92cd-4b3eee61c6c9
relation.isAuthorOfPublication596986f6-6f41-4107-a097-bc205ecdb95a
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublicatione9ec518c-41f4-4d7a-ad45-6eb2fae83693
relation.isAuthorOfPublication.latestForDiscovery596986f6-6f41-4107-a097-bc205ecdb95a
relation.isProjectOfPublicationa1a911dc-f170-4cc3-bdfa-770dbf2bb484
relation.isProjectOfPublication.latestForDiscoverya1a911dc-f170-4cc3-bdfa-770dbf2bb484

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Impact_CRCCalado.pdf
Size:
2.44 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEL - Eng. Quim. Biol. - Comunicações