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Cholesterol transporter proteins in HepG2 cells can be modulated by phenolic compounds present in Opuntia ficus-indica aqueous solutions

2020-01Journal article Restricted access
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dc.contributor.authorRESSAISSI, Asma
dc.contributor.authorATTIA, Nebil
dc.contributor.authorPacheco, Rita
dc.contributor.authorFale, Pedro
dc.contributor.authorSerralheiro, Maria Luisa
dc.date.accessioned2020-04-03T12:25:51Z
dc.date.available2020-04-03T12:25:51Z
dc.date.issued2020-01
dc.description.abstractIncreased blood cholesterol is a risk factor for atherosclerotic cardiovascular disease. This study tested the hypothesis that phenolic compounds can modulate the level of cholesterol transporters including Niemann-Pick C1-like 1 (NPC1L1) and ATP-binding cassette transporters in liver cells. HepG2 cells, used as a model of hepatocytes, showed a decrease in the abundance of cholesterol transporters comparatively to the control when treated with the Opuntia ficus-indica's cladodes decoction. The decrease was between 13-70%, 25-60%, 9-60% and 23-60% for NPC1L1, ABCA1, ABCG5 and ABCG8 transporters, respectively, when using between 0.15 and 0.35 mg/mL of decoction in the culture medium. FTIR analysis showed changes in the amount of RNA, which may be the cause of the decrease in the level of several proteins. These in vitro results pave the way to a molecular explanation for the decoction of cladodes effect on cholesterol levels as it reduced the membrane cholesterol transporter proteins, NPC1L1, ABCG5/ABCG8 and ABCA1, in HepG2 cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRESSAISSI, Asma; [et al] – Cholesterol transporter proteins in HepG2 cells can be modulated by phenolic compounds present in Opuntia ficus-indica aqueous solutions. Journal of Functional Foods. ISSN 1756-4646. Vol. 64 (2020), pp. 1-7pt_PT
dc.identifier.doi10.1016/j.jff.2019.103674pt_PT
dc.identifier.issn1756-4646
dc.identifier.urihttp://hdl.handle.net/10400.21/11399
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationUID/MULTI/04046/2019 - FCTpt_PT
dc.relationPTDC/BIA-BQM/28355/2017 - FCTpt_PT
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dc.subjectOpuntia ficus-indicapt_PT
dc.subjectCladodespt_PT
dc.subjectCholesterolpt_PT
dc.subjectNPC1L1pt_PT
dc.subjectATB-binding cassettespt_PT
dc.subjectFTIRpt_PT
dc.subjectPhenolic compoundspt_PT
dc.titleCholesterol transporter proteins in HepG2 cells can be modulated by phenolic compounds present in Opuntia ficus-indica aqueous solutionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage7pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Functional Foodspt_PT
oaire.citation.volume64pt_PT
person.familyNameRESSAISSI
person.familyNameATTIA
person.familyNamePacheco
person.familyNameFale
person.familyNameSerralheiro
person.givenNameAsma
person.givenNameNebil
person.givenNameRita
person.givenNamePedro
person.givenNameMaria Luisa
person.identifier1022927
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.orcid0000-0002-0073-6785
person.identifier.orcid0000-0002-9945-3143
person.identifier.orcid0000-0001-5192-3006
person.identifier.orcid0000-0002-4644-7655
person.identifier.orcid0000-0001-7541-9613
person.identifier.ridC-3062-2012
person.identifier.ridA-5570-2013
person.identifier.ridE-3407-2012
person.identifier.scopus-author-id8853708300
person.identifier.scopus-author-id25649775000
person.identifier.scopus-author-id6602688573
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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