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A nonionic porphyrin as a noninterfering DNA antibacterial agent

2011-11Journal article Restricted access
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dc.contributor.authorMendes, Sónia
dc.contributor.authorCamacho, Fábio
dc.contributor.authorSilva, Tito
dc.contributor.authorCalado, Cecília
dc.contributor.authorSerra, Arménio Coimbra
dc.contributor.authorGonsalves, António M. d’A. Rocha
dc.contributor.authorRoxo‐Rosa, Mónica
dc.date.accessioned2020-09-18T09:25:10Z
dc.date.available2020-09-18T09:25:10Z
dc.date.issued2011-11
dc.description.abstractThe increasing interest in clinical bacterial photodynamic inactivation has led to the search for photosensitizers with higher bactericidal efficiency and less side effects on the surrounding tissues. We present a novel nonionic porphyrin, the 5,10,15‐tris(2,6‐dichlorophenyl)‐20‐[4‐N‐(6‐amino‐hexyl)sulfonamido)phenyl]‐porphyrin (ACS769F4) with substantial improvements in the efficiency of nonionic sensitizers. This porphyrin causes eradication of both Escherichia coli and Staphylococcus aureus by the photodynamic effect but in higher concentrations compared with 5,10,15,20‐tetrakis (4‐N,N,N‐trimethylammoniumphenyl)‐porphyrin p‐tosylate (TTAP4+), a known bactericidal tetracationic porphyrin. More important, under such conditions, ACS769F4 proved to be harmless to two mammalian cells lines (human embryonic and baby hamster kidney), causing no reduction in their viability or negative impact on their cytoskeleton, despite its accumulation in cellular structures. On the contrary, TTAP4+ is shown to accumulate in the nucleus of mammalian cells, in association to DNA, causing chromatin condensation after exposure to light. Furthermore, dark incubation with TTAP4+ was shown to have a deleterious effect on the microtubule network. Based on its bactericidal efficiency, also observed without exposure to light, and on the low tendency to be harmful or genotoxic to mammalian cells, ACS769F4 should be looked at as an interesting photosensitizer to be evaluated for clinical purposes.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMENDES, Sónia; [et al] – A A nonionic porphyrin as a noninterfering DNA antibacterial agent. Photochemistry and Photobiology. ISSN 1751-1097. Vol. 87, N.º 6 (2011), pp. 1395-1404pt_PT
dc.identifier.doi10.1111/j.1751-1097.2011.00984.xpt_PT
dc.identifier.issn1751-1097
dc.identifier.urihttp://hdl.handle.net/10400.21/12244
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1751-1097.2011.00984.xpt_PT
dc.subjectNonionic porphyrinpt_PT
dc.subjectDNApt_PT
dc.subjectAntibacterial agentpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.titleA nonionic porphyrin as a noninterfering DNA antibacterial agentpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1404pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage1395pt_PT
oaire.citation.titlePhotochemistry and Photobiologypt_PT
oaire.citation.volume87pt_PT
person.familyNameCalado
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc

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