Repository logo
 
Publication

High-throughput bioassay for mechanism of action determination of antibacterial drugs

2017-03-30Conference object Restricted access
Simple item page
dc.contributor.authorRibeiro Da Cunha, Bernardo
dc.contributor.authorFonseca, Luís P. P.
dc.contributor.authorCalado, Cecília
dc.date.accessioned2017-11-29T16:01:41Z
dc.date.available2017-11-29T16:01:41Z
dc.date.issued2017-03-30
dc.description.abstractWhile the ‘war’ on infectious diseases has been considered won, antibiotic-resistant bacteria are currently responsible for 25,000 death’s yearly in Europe. No new broadspectrum antibiotic has been introduced since the 1960s, and the last new class was discovered in 1986. As the antibiotic pipeline is clearly exhausted, new tools to advance antibiotic research are required. The current work explored Fourier-transform infrared spectroscopy to classify the mechanism of action of 13 antibiotics, acting by 3 distinct Mode-Of-Action (MOA) and belonging to 7 different classes. After optimization of a biological assay and pre-processing techniques, principal component analysis and partial least squares discriminant analysis were applied in a multi-level approach, including the MOA, antibiotic class and ultimately individual antibiotics acting on very specific molecular targets. Overall results indicate that the proposed method presents metabolic resolution to identify antibiotics at three levels of classification (i.e. different MOA, classes and even acting on specific targets). Interestingly, the resolution capacity obtained at these three levels of classification depended on the antibiotic type, which highlights the importance of the multilevel approach taken. Ultimately the present work reinforces the applicability of the method has a metabolic fingerprinting tool for antibiotic discovery.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBERNARDO, Cunha; FONSECA, Luís; CALADO, Cecília - High-Throughput Bioassay for Mechanism of Action Determination of Antibacterial Drugs. In 2017 IEEE 5th Portuguese Meeting on Bioengineering. Coimbra, Portugal: IEEE, 2017. ISBN: 978-1-5090-4801-4. Pp. 1-4pt_PT
dc.identifier.doi10.1109/ENBENG.2017.7889478pt_PT
dc.identifier.isbn978-1-5090-4801-4
dc.identifier.urihttp://hdl.handle.net/10400.21/7629
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIEEE Xplorept_PT
dc.relation.publisherversionhttp://ieeexplore.ieee.org/document/7889478/pt_PT
dc.subjectAntibioticspt_PT
dc.subjectPrincipal component analysispt_PT
dc.subjectInhibitorspt_PT
dc.subjectMicroorganismspt_PT
dc.subjectProteinspt_PT
dc.subjectFilteringpt_PT
dc.titleHigh-throughput bioassay for mechanism of action determination of antibacterial drugspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F69242%2F2006/PT
oaire.citation.conferencePlaceCoimbra Portugal de fevereiro 16-18, 2017pt_PT
oaire.citation.endPage4
oaire.citation.startPage1
oaire.citation.title2017 IEEE 5th Portuguese Meeting on Bioengineeringpt_PT
oaire.fundingStream3599-PPCDT
person.familyNameRibeiro da Cunha
person.familyNameP. Fonseca
person.familyNameCalado
person.givenNameBernardo
person.givenNameLuis
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-idEA1E-4BEA-A01E
person.identifier.ciencia-id951D-DF38-3397
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-0303-9416
person.identifier.orcid0000-0001-8429-6977
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridP-6154-2017
person.identifier.ridA-4228-2013
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id57211629814
person.identifier.scopus-author-id55916288400
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication810fc4c7-6c05-44a0-81e5-6cfdb3c2088a
relation.isAuthorOfPublication5c533551-5113-4c0a-93f1-9c50cbd796bc
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc
relation.isProjectOfPublicatione934d4a3-07d0-4c5d-8183-e76b3b0bf237
relation.isProjectOfPublication.latestForDiscoverye934d4a3-07d0-4c5d-8183-e76b3b0bf237

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
High-Throughput Bioassay_C.Calado_ADEQ.pdf
Size:
960.22 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEL - Eng. Quim. Biol. - Comunicações