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Effect of tween-80 on stability and secretion of hydrophobic tagged-cutinases

2009Journal article Open access
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dc.contributor.authorCalado, Cecília
dc.contributor.authorBrandão, M.
dc.contributor.authorBiscaia, J.
dc.contributor.authorCabral, J. M. S.
dc.contributor.authorFonseca, L. P.
dc.date.accessioned2020-09-22T13:00:26Z
dc.date.available2020-09-22T13:00:26Z
dc.date.issued2009
dc.description.abstractTo significantly enhance the downstream processing, two cutinase variants were constructed by genetic fusion small hydrophobic peptides (WP)2 and (WP)4, respectively. However, the fusion of these peptides impairs cutinase secretion by the host cell Saccharomyces cerevisiae and increases cutinase inactivation in the culture broth due to cutinase aggregation, resulting in cutinase activities per biomass of 56 % of cutinase-(WP)2 and of 7 % of cutinase-(WP)4, in relation to cutinase without the hydrophobic tags. It was observed that the addition of non-ionic surfactant Tween-80 into the culture broth could minimise the cutinase inactivation. The addition of Tween-80 also results in the enhancement of cutinase secretion by the yeast cell, leading to 1.25 and 2.51 fold-higher extracellular cutinase-(WP)2 and cutinase-(WP)4, respectively, in relation to cultivations performed in the absence of surfactant. Therefore, the addition of Tween-80 on the culture broth partly minimises the effect of fusion of the hydrophobic tags on the inactivation of the enzymatic activity and on the reduction of the protein secretion. By this way, the use of Tween-80 on the S. cerevisiae cultivation may contribute to the efficiency enhancement of the downstream processing of tagged cutinases.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCALADO, Cecíla R. C.; [et al] – Effect of tween-80 on stability and secretion of hydrophobic tagged-cutinases. Chemical and Biochemical Engineering Quarterly. ISSN 0352-9568. Vol. 23, N.º 4 (2009), pp. 411-417pt_PT
dc.identifier.doi10.15255/CABEQ.2014.269pt_PT
dc.identifier.issn0352-9568
dc.identifier.issn1846-5153
dc.identifier.urihttp://hdl.handle.net/10400.21/12249
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherUniversity of Zagrebpt_PT
dc.subjectCutinasept_PT
dc.subjectTagged-cutinasept_PT
dc.subjectTween-80pt_PT
dc.subjectSecretionpt_PT
dc.titleEffect of tween-80 on stability and secretion of hydrophobic tagged-cutinasespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage417pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage411pt_PT
oaire.citation.titleChemical and Biochemical Engineering Quarterlypt_PT
oaire.citation.volume23pt_PT
person.familyNameCalado
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc

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