Repository logo
 
Publication

A flow injection analysis system for onā€line monitoring of cutinase activity at outlet of an expanded bed adsorption column almost in real time

2006-10Journal article Restricted access
Simple item page
dc.contributor.authorAlmeida, ClƔudio F.
dc.contributor.authorCalado, CecĆ­lia
dc.contributor.authorBernardino, Susana A.
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorFonseca, LuĆ­s P. P.
dc.date.accessioned2020-09-22T16:27:49Z
dc.date.available2020-09-22T16:27:49Z
dc.date.issued2006-10
dc.description.abstractExpanded bed adsorption (EBA) was used to recover, concentrate and purify Fusarium solani pisi cutinase, secreted by a recombinant Saccharomyces cerevisiae strain, directly from a whole fermentation culture. A flow injection analysis (FIA) system for monitoring Fusarium solani pisi cutinase based on microencapsulation of pā€nitrophenylbutyrate (pā€NPB) in a micellar system (sodium cholate, tetrahydrofuran, phosphate buffer) was developed for monitoring this target enzyme at the outlet tube of the EBA column. Slight differences in yeast cultivation conditions during cutinase production may influence the fermentation performance, which affects directly the adsorption of cutinase during the loading step and consequently the efficiency of the EBA process. This effect can be especially relevant when it is necessary to stop the application of feedstock to the EBA column when the outlet concentration (A) of the desired product is lower than 5% of the feed concentration (Ao). Excellent correlations between the FIA system and the offā€line analytical method for monitoring cutinase activity during the different EBA steps were obtained. Additionally, the blocking/fouling of the sample injector and tubes of the FIA system initially observed were eliminated due to the excellent surfactant properties of the sodium cholate contained in the phosphate buffer and used to dilute the enzyme samples. This FIA system was shown to be a powerful analytical tool for monitoring cutinase activity almost in real time (45ā€“60 s), maximizing enzyme adsorption while minimizing product loss and consequently maximizing the recovery yield of the product. Copyright Ā© 2006 Society of Chemical Industrypt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationALMEIDA, ClĆ”udio F.; [et al] ā€“ A flow injection analysis system for onā€line monitoring of cutinase activity at outlet of an expanded bed adsorption column almost in real time. Journal of Chemical Technology and Biotechnology. ISSN 1097-4660. Vol. 81, N.Āŗ 10 (2006), pp. 1678-1684pt_PT
dc.identifier.doi10.1002/jctb.1587pt_PT
dc.identifier.issn1097-4660
dc.identifier.urihttp://hdl.handle.net/10400.21/12251
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationPRAXIS XXI program, MinistĆ©rio da CiĆŖncia e Tecnologia, Portugal. Grant Number: BM/19093/99pt_PT
dc.relationPRAXIS XXI program, MinistĆ©rio da CiĆŖncia e Tecnologia, Portugal. Grant Number: BD/18276/98pt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/jctb.1587pt_PT
dc.subjectOnā€line monitoringpt_PT
dc.subjectControlpt_PT
dc.subjectExpanded bed adsorption (EBA)pt_PT
dc.subjectCutinasept_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectMicellar flow injection analysis systempt_PT
dc.subjectUnding Informationpt_PT
dc.titleA flow injection analysis system for onā€line monitoring of cutinase activity at outlet of an expanded bed adsorption column almost in real timept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1684pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage1678pt_PT
oaire.citation.titleJournal of Chemical Technology and Biotechnologypt_PT
oaire.citation.volume81pt_PT
person.familyNameCalado
person.familyNameCabral
person.familyNameP. Fonseca
person.givenNameCecĆ­lia
person.givenNameJoaquim M.S.
person.givenNameLuis
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-idAB13-9ED3-C821
person.identifier.ciencia-id951D-DF38-3397
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-2405-5845
person.identifier.orcid0000-0001-8429-6977
person.identifier.ridE-2102-2014
person.identifier.ridG-2052-2010
person.identifier.ridA-4228-2013
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id7201350203
person.identifier.scopus-author-id55916288400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication596986f6-6f41-4107-a097-bc205ecdb95a
relation.isAuthorOfPublication5c533551-5113-4c0a-93f1-9c50cbd796bc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
A flow_CRCCalado.pdf
Size:
196.17 KB
Format:
Adobe Portable Document Format
Download

Collections

ISEL - Eng. Quim. Biol. - Artigos