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Potential of FTIR-spectroscopy for drugs screening against Helicobacter pylori

2020-12-12Journal article Open access
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dc.contributor.authorSampaio, Pedro
dc.contributor.authorCalado, Cecília
dc.date.accessioned2020-12-16T12:23:44Z
dc.date.available2020-12-16T12:23:44Z
dc.date.issued2020-12-12
dc.descriptionEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2016 do Instituto Politécnico de Lisboa. Código de referência IPL/2018/RENALPROG_ISEL
dc.descriptionEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2016 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL
dc.descriptionEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2016 do Instituto Politécnico de Lisboa. Código de referência IPL/2020/NephroMD/ISEL
dc.description.abstractHelicobacter pylori colonizes the human stomach of half of the world’s population. The infection if not treated, persists through life, leading to chronic gastric inflammation, that may progress to severe diseases as peptic ulcer, gastric adenocarcinoma, and mucosa-associated lymphoid tissue lymphoma. The first line of treatment, based on 7 to 21 days of two antibiotics associated with a proton pump inhibitor, is, however, already failing most due to patient non-compliance that leads to antibiotic resistance. It is, therefore, urgent to screen for new and more efficient antimicrobials against this bacterium. In this work, Fourier Transform Infrared (FTIR) spectroscopy was evaluated to screen new drugs against H. pylori, in rapid (between 1 to 6 h), and high-throughput mode and based on a microliter volume processes in relation to the agar dilution method. The reference H. pylori strains 26,695 and J99, were evaluated against a peptide-based antimicrobial and the clinical antibiotic clarithromycin, respectively. After optimization of the assay conditions, as the composition of the incubation mixture, the time of incubation, and spectral pre-processing, it was possible to reproducibly observe the effect of the drug on the bacterial molecular fingerprint as pointed by the spectra principal component analysis. The spectra, obtained from both reference strains, after its incubation with drugs concentrations lower than the MIC, presented peak ratios statistically different (p < 0.05) in relation to the bacteria incubated with drugs concentrations equal or higher to the MIC. It was possible to develop a partial least square regression model, enabling to predict from spectra of both bacteria strains, the drug concentration on the assay, with a high correlation coefficient between predicted and experimental data (0.91) and root square error of 40% of the minimum inhibitory concentration. All this points to the high potential of the technique for drug screening against this fastidious growth bacterium.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSAMPAIO, Pedro S.; CALADO, Cecília R. C. – Potential of FTIR-spectroscopy for drugs screening against Helicobacter pylori. Antibiotics. ISSN 2079-6382. Vol. 9, N.º 12 (2020), pp. 1-15pt_PT
dc.identifier.doi10.3390/antibiotics9120897pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10400.21/12488
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationProjeto financiado no âmbito do Concurso de Projetos de Investigação, Desenvolvimento, Inovação & Criação Artística (IDI&CA) financiados pelo Instituto Politécnico de Lisboa. IPL/2017/DrugsPlatf/ISELpt_PT
dc.relationProjeto financiado no âmbito do Concurso de Projetos de Investigação, Desenvolvimento, Inovação & Criação Artística (IDI&CA) financiados pelo Instituto Politécnico de Lisboa. IPL/2018/RENALPROG_ISELpt_PT
dc.relationProjeto financiado no âmbito do Concurso de Projetos de Investigação, Desenvolvimento, Inovação & Criação Artística (IDI&CA) financiados pelo Instituto Politécnico de Lisboa. IPL/2020/NephroMD/ISELpt_PT
dc.subjectDrugs screeningpt_PT
dc.subjectFourier-transform infrared (FTIR) spectroscopypt_PT
dc.subjectHelicobacter pyloript_PT
dc.subjectHigh-throughput screeningpt_PT
dc.titlePotential of FTIR-spectroscopy for drugs screening against Helicobacter pyloript_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage15pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume9pt_PT
person.familyNameSampaio
person.familyNameCalado
person.givenNamePedro
person.givenNameCecília
person.identifier1251979
person.identifier130332
person.identifier.ciencia-idAF12-6ABA-43D8
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0003-2917-4904
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id24178064100
person.identifier.scopus-author-id6603163260
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication303f3e22-ec1a-4243-9ded-98c647776e6a
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc

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