Repository logo
 
Publication

Modelling, monitoring and control of plasmid bioproduction in Escherichia coli cultures

2012-10-18Conference object Open access
Simple item page
dc.contributor.authorLopes, Marta B.
dc.contributor.authorScholtz, Teresa
dc.contributor.authorSilva, Daniel
dc.contributor.authorSantos, Inês
dc.contributor.authorSilva, Tito
dc.contributor.authorSampaio, Pedro
dc.contributor.authorCouto, Andreia
dc.contributor.authorLopes, Vitor V.
dc.contributor.authorCalado, Cecília
dc.date.accessioned2020-07-30T15:52:43Z
dc.date.available2020-07-30T15:52:43Z
dc.date.issued2012-10-18
dc.description.abstractAn integrated approach for modelling, monitoring and control the plasmid bioproduction in Escherichia coli cultures is presented. In a first stage, by the implementation of a kinetic model for E. coli cultures, a better bioprocess understanding was reached, concerning the availability of nutrients and products along the bioprocess, and their effects on the plasmid production. Results presented may provide significant help for future modelling and monitoring implementation. In a second stage, FTIR spectroscopy coupled with chemometrics, namely PLS regression, shows its potential as a high-throughput technique for simultaneously estimating the key variables involved in the plasmid production process by E. coli cultures run under distinct conditions. Finally, owing to online monitoring and process control, an NIR fibre optic probe and chemometrics provided promising results concerning the control of biomass and carbon sources in E. coli cultures.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLOPES, Marta B.; [et al] – Modelling, monitoring and control of plasmid bioproduction in Escherichia coli cultures. In 2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG). Coimbra, Portugal: IEEE, 2012. ISBN 978-1-4673-4524-8. Pp. 1-6pt_PT
dc.identifier.doi10.1109/ENBENG.2012.6331370pt_PT
dc.identifier.isbn978-1-4673-4524-8
dc.identifier.isbn978-1-4673-4526-2
dc.identifier.urihttp://hdl.handle.net/10400.21/12122
dc.language.isoengpt_PT
dc.publisherInstitute of Electrical and Electronics Engineerspt_PT
dc.relationPTDC/BIO169242/2006 - FCTpt_PT
dc.relation.publisherversionhttps://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6331370&tag=1pt_PT
dc.subjectPlasmidpt_PT
dc.subjectMonitoringpt_PT
dc.subjectModellingpt_PT
dc.subjectControlpt_PT
dc.subjectFourier transform infraredpt_PT
dc.subjectNear-infraredpt_PT
dc.subjectSpectroscopypt_PT
dc.subjectEscherichia colipt_PT
dc.titleModelling, monitoring and control of plasmid bioproduction in Escherichia coli culturespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F73758%2F2010/PT
oaire.citation.conferencePlace23-25 Feb. 2012 - Coimbra, Portugalpt_PT
oaire.citation.endPage6pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.title2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)pt_PT
oaire.fundingStreamSFRH
person.familyNameCalado
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc
relation.isProjectOfPublication2e866b8b-53df-4729-b132-92adf509db2c
relation.isProjectOfPublication.latestForDiscovery2e866b8b-53df-4729-b132-92adf509db2c

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Modelling_CRCCalado.pdf
Size:
163.8 KB
Format:
Adobe Portable Document Format
Download

Collections

ISEL - Eng. Quim. Biol. - Comunicações